高效液相色谱法研究3-正丁基苯酞在大鼠体内的组织分布情况  

Study on the Tissue Distribution of 3-n-Butylphthalide in Rats by HPLC

作　　者：王宁宁[1] 李晓红[1] 李月[1] 姜明燕[1] 

机构地区：[1]中国医科大学附属第一医院药学部,沈阳110001

出　　处：《中国药学杂志》2012年第19期1574-1577,共4页Chinese Pharmaceutical Journal

摘　　要：目的研究3-正丁基苯酞(NBP)在大鼠体内的组织分布。方法采用Hypersil ODS2色谱柱(4.6 mm×250 mm,5μm),流动相为0.05 moL.L-1醋酸钠溶液-乙睛(50∶50,用醋酸调节pH 4.5),检测波长228 nm,柱温40℃。大鼠按3-正丁基苯酞混悬液单剂量360 mg.kg-1灌胃给药,采用高效液相色谱法测定各组织中3-正丁基苯酞的含量。结果 3-正丁基苯酞在各组织样品中的线性范围均为0.049 8～9.96μg.mL-1,线性关系良好。给药后1 h在肾中3-正丁基苯酞的浓度最高,其次是脑、心和肝。结论该方法灵敏度高,专属性强,结果准确可靠,适合用于研究3-正丁基苯酞在大鼠中的组织分布。研究表明,3-正丁基苯酞在大鼠体内分布快而广,且可通过血脑屏障。OBJECTIVE To study the tissue distriburion of 3-n-butylphthalide （NBP） in rats. METHODS NBP was analyzed on a ttypersil ODS2 column with the mobile phase consisting of 0. 05 moL· L-1 sodium acetate-acetonitrile （50： 50, adjust pH to 4. 5 with acetic acid）. The flow rate was 1.0 mL·min-1 and the column temperature was 40℃. The wavelength of UV detector was set at 228 nm. A single dose of 360 mg· kg-1 NBP suspensions was administered orally to rats. The tissues of heart, brain, liver and kidney were taken at 1, 2, 4 and 8 h after administration. NBP concentrations in tissues were assayed by HPLC-UV method. RESULTS The calibration curves for NBP in tissues were obtained over the concentration range of 0. 049 8 - 9.96 μg· mL -1 with good linearity and recoveries. The concentration of NBP at 1 h after administration was maximum in kidney followed by brain, heart and liver. CON- CLUSION The method is simple, sensitive, specific, accurate and stable. It can be used for the study on tissue distribution of NBP in rats. The results show that NBP is rapidly and widely distributed in rats and can cross the blood-brain barrier.

关 键 词：3-正丁基苯酞 分布 高效液相色谱法 

分 类 号：R965[医药卫生—药理学]