牛流行热病毒山东流行株G基因的克隆及序列分析  

Cloning and Sequence Analysis of the G Gene of Bovine Ephemeral Fever Virus Strains in Shandong Province

作　　者：侯佩莉[1] 杨宏军[1] 王洪梅[1] 刘文浩[1] 何洪彬[1] 

机构地区：[1]山东省农业科学院奶牛研究中心,山东济南250100

出　　处：《动物医学进展》2012年第10期1-5,共5页Progress In Veterinary Medicine

基　　金：泰山学者海外特聘专家专项经费(何洪彬);国家奶牛产业技术体系建设专项经费(何洪彬);山东省科技攻关项目(2009GG20002032);济南市高校院所自主创新计划(201004027);转基因重大专项(2011ZX08007-002;2011ZX08008-004)

摘　　要：为了解牛流行热病毒(BEFV)山东流行株G基因的特点,从临床病料中获得山东流行株牛流行热病毒的G基因,并对其进行序列测定和分析。参考GenBank中牛流行热病毒的G基因序列,设计并合成一对特异性扩增G基因引物进行PCR,扩增目的片段,并克隆于pEASY-T3载体上,筛选阳性重组质粒进行序列测定,用MEGA软件、NJ法构建进化树。结果显示,该流行毒株G基因与GenBank中其他BEFV株G基因的核苷酸推导氨基酸的同源性均大于96%;遗传进化关系分析结果表明,BEFV山东流行株与中国台湾流行株具有较高同源性和较近的亲缘关系。To study the character of G gene of bovine ephemeral fever virus strains circulating in Shandong province, cloning and sequence analysis of the G gene obtained from clinical specimens were conducted. A pair of primers of G gene were designed and synthesized on the basis of bovine ephemeral fever virus published in GenBank, and the sequence was amplified from Shandong strains isolate by PCR. The PCR products were cloned into T vector and then sequenced positive recombinant plasmids. Phylogenetic tree was constructed by the Neighbor joining method with MEGA soft ware. Comparison of this sequence with that of G gene of bovine ephemeral fever virus in GenBank, the results of nucleotide sequence and amino acid homology were above 96 % ,and evolutionary processes analyses showed that this strain had a close relationship with China Taiwan strain.

关 键 词：牛流行热病毒 山东流行株 G基因 序列分析 

分 类 号：S852.653[农业科学—基础兽医学]