CRISPR系统结构与功能研究进展  

Progress on Structure and Function of CRISPR System

作　　者：蒋伟[1] 黎满香[1] 田世成[1] 颜运秋[1] 

机构地区：[1]湖南农业大学动物医学院,湖南长沙410128

出　　处：《动物医学进展》2012年第10期82-86,共5页Progress In Veterinary Medicine

基　　金：湖南省科技计划项目(2010NK3015)

摘　　要：自然界中的微生物总是通过各种各样的保护机制,使它们能够与恶劣的环境及侵袭性核酸共存。许多微生物都能通过基于基因序列特异性的方式抵抗核酸入侵。CRISPR(clustered regularly inter-spaced short palindromic repeats)即是属于这种保护机制之一。该位点是由独特的DNA间区间隔开的短的重复序列组成,并与一些被称为CRISPR相关蛋白(CRISPR association protein)的蛋白质共同作用,形成一套完整的自我保护机制。这种高变位点能从外源片段中摄取约36bp的短片段,从而建立可遗传的、DNA介导的"特异性免疫保护机制"。Bacteria can protect them from bad environment and invading nucleic acid by various protection mechanisms in nature. Most bacteria have the self-defense mechanisms mediated by specific genes. A genetic locus termed CRISPR （clustered regularly interspaced short palindromic repeats） is exit in many bacteria and archaea, which can provide bacteria from plasmid and invading acid though sequence-specificity. CRISPR consists of short repeat interspersed with unique DNA spacers, and adjacent CRISPR-associated protein encoding genes. CRISPR loci can take up foreign nucleic acid elements to build the genetic and DNA-mediated ＂specific immune protection mechanism＂.

关 键 词：CRISPR位点 噬菌体 质粒 外源基因片段 

分 类 号：S852.61[农业科学—基础兽医学]