以长白猪为受体母猪生产克隆五指山小型猪的妊娠诊断  

Pregnancy Diagnosis of Landrace as Surrogate Sows Generating Cloned Wuzhishan Minipigs

作　　者：王伟[1] 崔成哲 李秀元 王晓明 尹熙俊[1] 李文学[1] 

机构地区：[1]延边大学农学院,吉林延吉133002 [2]延边农业科学院,吉林龙井133400

出　　处：《动物医学进展》2012年第10期117-120,共4页Progress In Veterinary Medicine

基　　金：国家转基因重大专项资金支持项目(2009ZX08006-002B)

摘　　要：采用B超结合猪早孕检测试剂盒对受体母猪进行妊娠诊断,确定了克隆五指山小型猪早期用B超进行妊娠诊断的最佳时间为胚胎移植后30d,可以检测到孕体和胎体反射;52d可以检测到胎儿的数量、胎儿骨骼及头部和脊柱;87d检测到胎动。在整个妊娠期采用B超联合猪早孕检测试剂盒进行妊娠诊断,能够及时排除干扰,提高克隆猪受体妊娠诊断的准确率。In this study, the real-time B-mode （brightness mode） ultrasonography associated with the early pregnancy diagnosis kit was used for pregnancy diagnosis in surrogate sows. The best time for early pregnancy diagnosis in cloned Wuzhishan minipigs was the 30th day after embryo transfer, when the gestational sac and carcass reflection can be detected; on the 52th day after embryo transfer, the number of fetuses, fetal bones, head and spine can be detected. Also on the 87th day the fetal movement can be detected. In addition, throughout the course of pregnan cy, the use of real-time B-mode （brightness mode） ultrasonography associated with early pregnancy diagnosis kit for pregnancy diagnosis in surrogate sows can timely eliminate interference, improve the diagnostic accuracy of pregnancy in cloned Wuzhishan minipigs.

关 键 词：克隆猪胚 母猪 B超 妊娠诊断 

分 类 号：S854.41[农业科学—临床兽医学]