增量表达Bmlipase-1的转基因家蚕的主要经济性状调查  

An Investigation on Main Traits of Transgenic Silkworm Overexpressing Bmlipase-1

作　　者：蒋亮[1] 孙强[2] 金盛凯[1] 徐美茜[2] 程廷才[1] 夏庆友[1,2] 

机构地区：[1]西南大学,家蚕基因组生物学国家重点实验室,重庆400715 [2]西南大学生物技术学院,重庆400715

出　　处：《蚕业科学》2012年第5期822-824,共3页ACTA SERICOLOGICA SINICA

基　　金：国家重点基础研究发展计划“973”项目(No.2012CB-114600);国家高技术研究发展计划“863”项目(No.2011-AA100306)

摘　　要：家蚕核型多角体病毒(BmNPV)是对养蚕业危害最严重的病原之一,采用传统育种方法培育家蚕抗性品种存在经济性状和抗性呈负相关的矛盾。为了确定转基因方法能否突破该瓶颈,对先前利用家蚕品种大造建立的增量表达内源性抗病毒基因Bmlipase-1的转基因家蚕系统LI-A的主要经济性状进行调查。结果显示,LI-A的4、5龄幼虫逐日体质量及产茧量性状成绩与正常大造之间没有明显的差异,表明转基因抗病毒家蚕系统LI-A在抗性提高的同时,经济性状没有受到影响。Bombyx mori nucleopolyhedrovirus （BmNPV） is one of the most serious pathogens undermining sericultural production. A negative correlation was found between economic trait and disease resistance in the resistant silkworm va- rieties obtained by traditionar breeding methods. To determine whether this bottleneck could be broken by transgenic technology, we investigated the main economic traits of LI-A, a transgenic silkworm lineage established from silkworm variety Dazao and being capable of overexpressing endogenous antiviral gene Bmlipase-1. The results showed that there were no obvious differences in daily body weight of the 4th and 5th instar larvae and in cocooning traits between LI-A and normal Dazao, revealing that the resistance of antiviral transgenic silkworm lineage LI-A was increased while the econom- ic traits were not affected.

关 键 词：家蚕核型多角体病毒 Bmlipase-1 转基因 抗性 经济性状 家蚕 

分 类 号：S882[农业科学—特种经济动物饲养]