碳糊电极阳极吸附伏安法测定中成药及保健品中的槲皮素  

Anodic Adsorptive Voltammetric Determination of Quercetin in Chinese Patent Medicine and Health-Care Product at A Carbon Paste Electrode

作　　者：周清[1] 温金莲[1] 朱明芳[1] 龙宁[1] 翟海云[1] 

机构地区：[1]广东药学院药科学院,广州510006

出　　处：《中国实验方剂学杂志》2012年第19期82-84,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广东省科技计划项目(2009198);国家自然科学基金项目(21005021)

摘　　要：目的:建立碳糊电极(CPE)阳极吸附伏安法测定中成药及保健品中槲皮素的方法。方法:用石墨粉-液体石蜡(4:1)制备CPE,在HAc-NaAc(pH 3.6)缓冲液中,槲皮素在0.401 V(vs.Ag-AgCl)产生一灵敏的吸附氧化峰。结果:槲皮素浓度在3.13×10-7～1.0×10-5mol.L-1与峰电流呈良好的线性关系(r=0.996 0),检出限为2.1×10-8mol.L-1,应用该法测定中成药及保健品中的槲皮素取得了较好的结果。结论:该法准确可靠、经济实用,可用于槲皮素的测定。Objective：To establish the determination method of quercetin in Chinese patent medicine and health-care product by carbon paste electrode（CPE）anodic adsorptive voltammetry.Method：The CPE was prepared by carbon powder and liquid paraffin（4∶1）,in a acetic acid-sodium acetate buffer solution（pH 3.6）.Quercetin gives a sensitive adsorptive oxidation peak at 0.401 V（vs.Ag-AgCl）.Result：The linear relationship between the peak current and the concentration was found between 3.13×10-7-1.0×10-5mol · L-1 with correlation coefficient of 0.996 0,and the detection limit was 2.1×10-8mol · L-1.The method was applied to the analysis of Chinese patent medicine and health-care product and has achieved good results.Conclusion：The method is accurate and reliable,economical and practical.

关 键 词：槲皮素 阳极吸附伏安法 碳糊电极 银杏叶片 槲皮素复合胶囊 

分 类 号：R284.1[医药卫生—中药学]