三拗汤对哮喘小鼠变应性气道炎症的影响及其成分分析  

Effects of Sanao Decoction on Allergic Airway Inflammation in Asthma Mouse and its Component Analysis

作　　者：马春华[1] 汪姗姗[2] 马世平[1] 范欣生[2] 

机构地区：[1]中国药科大学中药药理教研室,南京210009 [2]南京中医药大学江苏省方剂研究重点实验室,南京210046

出　　处：《中国实验方剂学杂志》2012年第19期149-153,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81072748);"十一五"国家科技支撑计划(2006BAI06A02);江苏高校优势学科建设工程项目(PAPD)

摘　　要：目的:评价三拗汤对哮喘小鼠变应性气道炎症的影响,并对全方成分进行分析。方法:BALB/c小鼠60只,随机分为正常组、模型组、三拗汤高、中、低剂量(7.2,3.6,1.8 g.kg-1)组、地塞米松组(0.75 mg.kg-1)。各组小鼠(除空白组)于第1,8天腹腔、皮下分别给予0.1 mL的致敏液(0.2 mL致敏液含卵蛋白OVA 0.1 mg,Al(OH)30.02 mg),第15～28天各组(除空白组)给予5%的OVA雾化,每次雾化20 min,每次雾化前30 min各组小鼠按体重给予相应的药物;空白组以等体积生理盐水代替致敏液,且生理盐水雾化相同的时间。相应的药物治疗2周后,取血做嗜酸细胞计数(EOS),取肺泡灌洗液(BALF)做细胞分类计数。采用超高效液相-四级杆串联飞行时间质谱(UPLC-QTOFMS)鉴定其化学成分。结果:三拗汤中剂量组使哮喘小鼠血中EOS的含量明显的降低(P<0.01),三拗汤高、低剂量组使哮喘小鼠血中的EOS含量有所降低(P<0.05),三拗汤高、中、低剂量使BALF中的EOS明显下降(P<0.01,P<0.05),从三拗汤全方中初步鉴定了22个化合物。结论:三拗汤对哮喘小鼠变应性气道炎症有明显的抑制作用,其全方主要化学成分为生物碱、黄酮、皂苷成分,这些化学组分与其发挥抗哮喘小鼠变应性气道炎症有密切的关系。Objective：To investigate the effects of Sanao decoction on allergic airway inflammation in asthma mouse and its component analysis.Method： Sixty BALB/c mice were divide into six groups：normal group,model group,Sanao decoction high,middle,low dose（7.2,3.6,1.8 g · kg-1）,positive group（0.75 mg ·kg-1）,mice in each group（except those in the normal group） were sensitized with 0.2 mL sensitized solution[0.2 mL sensitized solution contains 0.1mg OVA and 0.02 mg Al（OH）3] during the period from 15 to 28 day,and given corresponding drugs 30 min later,mice were atomized with 5% OVA;mice in normal group were sensitized with physiological saline and were atomized with physiological saline.Samples ofblood were collected for counting numbers of eosinophilic granulocyte（EOS）,and broncho alveolar laverge fluid（BALF）was colledted for counting inflammatory cells.UPLC-TOFMS was used to analyze its chemical compositions.Result： Sanao decoction could obviously reduce numbers of EOS（P0.01,P0.05）,in blood and BALF（P0.05,P0.01） and 22 compounds in Sanao decoction could be identified.Conclusion： Sanao decoction can obviously inhibit allergic airway inflammation in asthma mouse,22 compounds which are related to effect of Sanao decoction are belong to alkaloids,flavonoids,saponins.

关 键 词：三拗汤 变应性气道炎症 成分分析 

分 类 号：R285.5[医药卫生—中药学]