复方雷公藤颗粒对再障小鼠骨髓T细胞亚群的影响  

Effect of Compound Tripterygium Granule on Bone Marrow T Cell Subsets in Mice Aplastic Anemia Model

作　　者：邢海燕[1] 胡春萍[1] 蔡雪婷[1] 胡灿红[1] 霍介格[1] 曹鹏[1] 王志刚[1] 

机构地区：[1]江苏省中医药研究院,南京210028

出　　处：《中国实验方剂学杂志》2012年第19期162-165,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江苏省中医药科技项目立项计划(LB09060)

摘　　要：目的:观察复方雷公藤颗粒对再生障碍性贫血(简称再障)小鼠的疗效及对骨髓CD4+,CD8+,CD4+/CD8+T淋巴细胞表达的影响。方法:建立再障小鼠模型,分为5组,正常组及模型组小鼠(给予同体积的生理盐水ig),复方雷公藤低、高剂量组(分别为生药10.63,13.29 g.kg-1,ig)、十一酸睾酮(22 mg.kg-1,ig),连续给药35 d,取骨髓单个核细胞培养24 h后,流式细胞术检测CD4+,CD8+,CD4+/CD8+T淋巴细胞的表达水平。结果:模型组CD4+,CD8+,CD4+/CD8+各指标均较正常组下降,其中CD4+,CD4+/CD8+T淋巴细胞明显低于正常组(P<0.05);十一酸睾酮组与模型组相比,CD4+,CD8+均有所下降,但CD4+/CD8+T细胞较之上升,但统计学均无明显差异,复方雷公藤颗粒低、高剂量组与模型组相比,CD4+,CD8+,CD4+/CD8+均升高,其中CD4+,CD4+/CD8+升高有统计学意义(P<0.05),与十一酸睾酮组相比差异有统计学意义(P<0.05)。结论:复方雷公藤颗粒能调节骨髓T细胞的异常状态,恢复T细胞及其亚群之间的平衡,促进骨髓造血功能的恢复。Objective：To observe the effect of Tripterygium granule on expression of bone marrow CD4＋/CD8＋T-lymphocytes in mice aplastic anemia model.Method：A mouse model of aplastic anemia was established and divided into five groups,normal group and model group（fed with normal saline）,compound Tripterygium granule group（10.63,13.29 g · kg-1,ig）,testosterone undecanoate group（22 mg · kg-1,ig）.After continuous administration of 35 d,bone marrow mononuclear cells were separated and cultured for 24 h.Expression levels of CD4＋,CD8＋,CD4＋/CD8＋T cells were analyzed by flow cytometry.Result： Compared with the normal group,amount of CD4＋,CD8＋,CD4＋/CD8＋T cells was decreased in model group,which of CD4＋,CD4＋/CD8＋T cells were significantly lower than the normal group（P0.05）,CD4＋and CD8＋were decreased,while CD4＋/CD8＋was increased in testosterone undecanoate group,but there was no significant difference compared with model group.Compared with the model group,amount of CD4＋,CD8＋,CD4＋/CD8＋in high and low dose group of compound Tripterygium granule was increased,which of CD4＋,CD4＋/CD8＋T cells was significantly different from model group and testosterone undecanoate group（P0.05）.Conclusion：Compound Tripterygium granule can regulate the abnormal state of bone marrow T cells,restore balance between T cells and T cell subsets,and promote the recovery of bone marrow function.

关 键 词：再生障碍性贫血 复方雷公藤颗粒 十一酸睾酮 T细胞亚群 

分 类 号：R285.5[医药卫生—中药学]