补阳还五汤含药血清对缺氧缺糖损伤PC12细胞形态和活力的影响  

Effects of Buyang Huanwu Decoction Containing Serum on the Morphology and Viability of PC12 Cells During Oxygen-glucose Deprivation

作　　者：刘俊娥[1,2] 张继平[2] 姚晖[2] 方永奇[3] 梁毅[3] 文凤妮[1] 

机构地区：[1]广东医学院药理学教研室,广东湛江524023 [2]南方医科大学附属佛山医院,广东佛山528000 [3]广州中医药大学第一附属医院实验中心,广州510405

出　　处：《中国实验方剂学杂志》2012年第19期186-190,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广东省科技厅科技攻关项目(2004B36001009);广东省中药局中医药强省课题(2010008)

摘　　要：目的:探讨补阳还五汤含药血清对缺氧缺糖(oxygen-glucose deprivation,OGD)损伤PC12细胞形态和活力的影响。方法:采用中药血清药理学方法制备补阳还五汤含药血清,以5%,10%,20%的含药血清作用于OGD损伤PC12细胞,于倒置显微镜下观察细胞形态,用四甲基偶氮唑盐法检测细胞活力。结果:PC12细胞OGD损伤后形态发生改变、细胞活力降低;5%,10%补阳还五汤含药血清作用于OGD损伤PC12细胞12,18,24 h,均可减轻细胞形态损伤、提高细胞活力(P<0.01);而20%补阳还五汤含药血清随作用时间的延长对OGD损伤PC12细胞的形态和活力具有双向调节作用。结论:一定浓度的补阳还五汤含药血清对OGD损伤PC12细胞的形态和活力具有保护作用。Objective：To investigate effects of Buyang Huanwu decoction containing serum on the morphology and viability of PC12 cells during oxygen-glucose deprivation.Method：Buyang Huanwu decoction containing serum was obtained by the seropharmacological method.PC12 cells were treated with different concentrations（5%,10%,20%） of the serum during oxygen-glucose deprivation.The cell morphology was observed with inverted microscopy,and the cell viability was examined by methyl thiazolyl tetrazolium assay.Result：The cell morphology was changed and the cell viability was decreased during oxygen-glucose deprivation,and the injured degree of cell morphology was relieved and the cell viability was increased in PC12 cells which treated with concentrations of 5%,10% Buyang Huanwu decoction medicated serum for 12,18,24 h（P0.01）.However,20% Buyang Huanwu decoction containing serum had the bidirectional regulation effects on the morphology and viability of PC12 cells during oxygen-glucose deprivation.Conclusion：The certain concentration of Buyang Huanwu decoction congtaining serum has protective effects on the morphology and viability of PC12 cells during oxygen-glucose deprivation.

关 键 词：补阳还五汤 含药血清 缺氧缺糖 PC12细胞 

分 类 号：R285.5[医药卫生—中药学]