龙胆碱对反复高热惊厥大鼠的解热抗惊厥作用机制探讨  

Mechanism Involved in Anticonvulsant Effect of Gentianine in Rats with Recurrent Febrile Seizures

作　　者：刘刚[1] 

机构地区：[1]连云港中医药高等职业技术学校中医药系,江苏连云港222007

出　　处：《中国实验方剂学杂志》2012年第19期235-237,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:探讨龙胆碱对反复高热惊厥大鼠的抗惊厥作用机制。方法:60只SD大鼠随机分为健康对照组、模型组、龙胆碱高、低剂量组,各15只。除健康对照组外,其余大鼠均按照文献方法制作高热惊厥大鼠模型。大鼠隔日诱导惊厥1次,共诱导10次,20 d。龙胆碱高、低剂量组每次惊厥后立即分别给予龙胆碱生理盐水溶液200,50 mg.kg-1,ip,模型组和健康对照组立即给予同体积生理盐水。观察各组大鼠惊厥潜伏期和惊厥持续时间;末次惊厥24 h后检测血清白细胞介素-1,6,8(IL-1,IL-6,IL-8),肿瘤坏死因子-α(TNF-α),干扰素-α(IFN-α)水平及脑组织匀浆中环磷酸腺苷(cAMP),前列腺素E2(PGE2)水平。结果:龙胆碱高、低剂量组大鼠惊厥潜伏期明显长于模型组(P<0.05);惊厥持续时间明显短于模型组(P<0.01)。(P<0.05);龙胆碱高、低剂量组血清IL-1,IL-6,IL-8,TNF-α,脑组织PGE2水平明显低于模型组(P<0.05)且高剂量组低于低剂量组(P<0.05)。结论:龙胆碱具有解热抗惊厥作用,其作用机制与体内细胞因子改变进而影响下丘脑PGE2水平相关。Objective：To investigate the mechanism involved in anticonvulsant effect of gentianine in rats with recurrent febrile seizures.Method：Sixty SD rats were randomly divided into the healthy control group,model group,gentianine high-dose（200 mg · kg-1） group,gentianine low-dose（50 mg · kg-1） group（n=15,each）.Except the health control group,all rats were induced high fever seizures according to the reference method.The rats were induced convulsion every other day,totally 10 times in 20 d.When convulsion happened,gentianine was injected（ip） immediately in the gentianine high-dose group and the low-dose group.In model group and health control group,the rats were given the same volume of normal saline.Convulsion latency and convulsion duration;serum IL-1,IL-6,IL-8 and TNF-α,IFN-α levels;brain homogenate cAMP and PGE2 level were investigated for the rats.Result：In gentianine groups,convulsions incubation period was significantly longer than that in the model group（P0.05）;convulsion duration was significantly less than that in the model group（P0.01）.In model group,IL-1,IL-6,IL-8,TNF-α and PGE2 levels were significantly higher than those in the control group and gentianine groups（P0.05）.In gentianine high-dose group,IL-1,IL-6,IL-8,TNF-α and PGE2 levels were significantly lower than those in gentianine low-dose group（P0.05）.Conclusion：Gentianine has antipyretic and anticonvulsant effect,which has relationship with cytokines affecting the hypothalamic PGE2 levels.

关 键 词：龙胆碱 惊厥 机制 

分 类 号：R285.5[医药卫生—中药学]