康心汤对异丙肾上腺素诱导实验性心肌肥厚大鼠维生素D代谢的调节作用  

Effect of Kangxin Decoction on Regulating Vitamin D in Rats with Myocardial Hypertrophy Induced by Isoproterenol

作　　者：陈云志[1] 杨长福[1] 郭军[2] 陈昱江[2] 王瑶瑶[2] 柴艺汇[3] 

机构地区：[1]贵阳中医学院基础医学院,贵阳550002 [2]贵阳中医学院第一临床医学院,贵阳550001 [3]贵阳中医学院研究生院,贵阳550002

出　　处：《中国实验方剂学杂志》2012年第19期257-260,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：贵州省科技厅科技基金[黔科合J字(2012)2064号];贵州省科技厅;贵阳中医学院联合基金[黔科合中药字(2010)LKZ7017];贵阳市科技局项目[筑科农合同字(2009)3-028];贵州省中医药管理局课题[QZYY2010-32]

摘　　要：目的:观察康心汤对异丙肾上腺素致大鼠心肌肥厚模型血清25羟维生素D3[25(OH)D3]的影响。方法:雄性SD大鼠随机分为5组:对照组,模型组,康心汤10,5,2.5 g.kg-1组。异丙肾上腺素2.4 mg.kg-1皮下注射,连续7 d,建立心肌肥厚模型,药物干预6周后酶联免疫法(ELISA)检测血清25(OH)D3,心房利钠肽(ANP),肾素(Renin),大鼠心肌标本HE染色观察组织病理改变。结果:与对照组比较,模组型血清25(OH)D3降低,ANP,Renin升高(P<0.05)。心肌HE染色见心肌排列紊乱,纤维化增生。与模型组比较,康心汤10 g.kg-1干预后,血清25(OH)D3升高,伴随ANP,Renin降低(P<0.05),心肌纤维化程度减轻。结论:康心汤对25(OH)D3的调节作用参与逆转异丙肾上腺素致大鼠的心肌肥厚。Objective：To observe the effect of Kangxin decoction on regulating vitamin D in rats with myocardial hypertrophy induced by isoproterenol.Method：Fifty male SD rats were randomly divided into 5 groups： normal group,model group,three dose groups of Kangxin decoction（10,5,2.5 g · kg-1）.Myocardial hypertrophy model in rats was induced by subcutaneous injection of isoproterenol（ISO,2.4 mg · kg-1,7 d）.After Kangxin Decoction intervention for 6 weeks,serum 25（OH） D3,atrial natriuretic peptide（ANP）,renin were detected by ELISA.Pathological changes in myocardial tissue were determined by hematoxylin-eosin（HE） staining.Result：Compared with the controls,serum 25（OH） D3 in the model group was reduced,and serum ANP and renin were elevated（P0.05）.Myocardium derangement and fibrotic hyperplasia were also seen in the models.After Kangxin decoction treatment,serum 25（OH） D3 was increased,accompanied with lower ANP and rennin.The degree of myocardial fibrosis was improved after the treatment.Conclusion：Kangxin decoction had an effective role to treat myocardial hypertrophy induced by ISO by regulationg vitamin D and protecting myocardial cells from injury.

关 键 词：心肌肥厚 维生素D 肾素 心房利钠肽 康心汤 

分 类 号：R285.5[医药卫生—中药学]