低剂量茶碱对气道黏液高分泌影响的研究  

作　　者：银春[1] 王利民[1] 孙勇[1] 邓丽娟[1] 刘春华[1] 欧阳莉[1] 

机构地区：[1]四川绵阳四0四医院呼吸内科,621000

出　　处：《浙江临床医学》2012年第10期1169-1172,共4页Zhejiang Clinical Medical Journal

基　　金：基金项目：四川省卫生厅（编号：110556）、绵阳市科技局（编号：1IM012）

摘　　要：目的探讨低剂量茶碱对大鼠气道黏液高分泌的影响及其作用机制。方法32只SD大鼠随机分为空白对照组、模型组、茶碱自身对照组、茶碱干预组，造模后第21天处死大鼠，采集肺组织标本和血标本，用苏木精－伊红（HE）染色、阿辛兰－过碘酸雪夫染色（AB－PAS），观察气道上皮细胞黏蛋白的分泌；免疫组化检测肺内气道杯状细胞的Muc5ac蛋白表达和肺内气道的核转录因子－κB（NF—κB）；实时荧光定量PCR（Real．timePCR）法检测肺组织Muc5acmRNA；放射免疫法检测肺组织匀浆cAMP含量；采用高效液相色谱法检测血浆茶碱浓度。结果丙烯醛雾化可导致大鼠出现明显的气道黏液高分泌表现，用低剂量茶碱干预可显著降低气道AB／PAS染色阳性、Muc5ac蛋白及mRNA的表达和NF－κB的入核率，但未能使肺组织cAMP含量恢复。结论低剂量茶碱可抑制丙烯醛所致的气道黏液高分泌，其机制可能与抑制NF－κB的活化有关；未显示通过抑制cAMP起降解作用。Objective To investigste the effect of low dose theophylline on airway mucous hypersecretion in rat model induced by inhaling acrolein and explore its mechanism. Methods The Sprague-Dawley （ SD ） rats were randomly assigned into 4 groups ： control group , theophylline self-control group , model group, theophylline intervention group .Lung tissue were acquired after the rats were sacrificed on the 21st day. Histopathology examination of lung tissue section stained with HE and AB-PAS. MucSac expression was detected by real-time PCR and immunohistochemisy.Nuclear factor- K B was assayed with immunohistochemisy. The level of cAMP of lung tissue was measured by radio-immunity. Concentration of theophylline in blood plasma was examined by high performance liquid chromatography. Results Acroline inhalation could upregulate the expression of MucSac, and it also increased the area ratio of AB/PAS staining on airway epithelial and nuclear translocation ratio of NF- K B. But the level of cAMP of lung tissue was decreased. After low dose theophylline intervention, all above-mentioned were obviously reduced except for the level of cAME Conclusion The results suggests that low dose theophylline can suppress airway mucus hypersecretioon, which is likely to act by acting NF- K B. It is not indicated that there is a relationship between mucus hypersecretioon and the level of cAME

关 键 词：丙烯醛 气道黏液高分泌 茶碱 MUC5AC 

分 类 号：R978.15[医药卫生—药品]