高效分子排阻色谱法同时测定白及多糖分子量和含量  

Determination of molecular weight and content of Bletilla striata glucomannan by high performance size exclusion chromatography

作　　者：李楠[1] 李卓 张燕[1] 王虎成[1] 刘江云[1] 蔡培烈[1,2] 杨世林[1] 

机构地区：[1]苏州大学药学院,苏州215123 [2]辽宁诺康生物制药有限责任公司,沈阳110016

出　　处：《药物分析杂志》2012年第10期1801-1803,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立同时测定白及多糖分子量和含量的高效分子排阻色谱分析方法。方法:采用TSK-gel G4000 PWXL色谱柱(10μm,7.8 mm×300 mm),流动相为纯水,流速0.6 mL·min-1,柱温30℃,示差折光检测器检测。结果:白及多糖重均分子量的线性范围为24.17～178.0 k(R2=0.9943),含量测定线性范围为8.22～20.55.mg·mL-1(R2=0.9992),分子量、含量精密度试验RSD分别为0.11%和0.96%(n=6),重复性RSD为0.28%和1.1%(n=5)。结论:该方法简便快速,结果准确,重复性好,可作为白及多糖分子量和含量测定的有效方法。Objective：To develop a high performance size exclusion chromatography method for the determination of molecular weight and content of polysaccharide from Bletilla striata.Methods：The TSK-gel G4000 PWXL（10 μm,7.8 mm× 300 mm） column was used at 30 ℃,with water as the mobile phase at the flow rate of 0.6 mL·min-1.Refractive index detector was applied.Results：The average molecular weight of Bletilla striata polysaccharide ranged from 24.17 k to 178.0 k（R2=0.9943）.The content of Bletilla striata polysaccharide ranged from 8.22 to 20.55 mg·mL-1（R2=0.9992）.Relative standard deviations（RSDs） of measurement precision of molecular weight and content were 0.11% and 0.96%（n=6）,respectively.RSDs of the repeatability were 0.28%,and 1.14%（n=5）,respectively.Conclusion：The method is simple,rapid and effective for the measurement of molecular weight and content of Bletilla striata polysaccharides.

关 键 词：白及多糖 分子排阻色谱(SEC) 分子量 含量 中药多糖分析 

分 类 号：R917[医药卫生—药物分析学]