离子色谱法测定硫酸小诺霉素中硫酸盐的含量  

Ion chromatography determination of the sulfate content in micronomicin sulfate

作　　者：张静 王庆全 刘绪平 

机构地区：[1]江西省食品药品检验所,南昌330029

出　　处：《药物分析杂志》2012年第10期1845-1846,共2页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立离子色谱法测定硫酸小诺霉素中硫酸盐的含量,为该类药质量控制标准提供参考。方法:色谱条件为:高容量氢氧化物选择性阴离子分析柱IonPac AS19(250 mm×4 mm)和IonPac AG19(50 mm×4 mm)保护柱,20 mmol·L-1氢氧化钾淋洗液以流速1.00 mL·min-1等度淋洗,配有连续自动再生微膜抑制器的电导检测器。结果:在选定的色谱条件下,方法检出限为4.95 ng;线性范围为9.9～69.3 mg·L-1,r=0.9992;平均加标回收率为99.2%～101.3%;精密度试验RSD为0.37%。结论:方法操作简单、快速,灵敏度高,结果准确,重复性好,可用于控制硫酸小诺霉素中硫酸盐的含量。Objective：To establish a new method to analyze the sulfate content in micronomicin sulfate.Methods：Ion chromatography was performed on a separation column of IonPac AS19（250 mm×4 mm）anion exchange column with a guard column of IonPac AG19（50 mm×4 mm）;20 mmol·L-1 KOH was used as the isocratic eluent,at a flow rate of 1.00 mL·min-1 with suppressed conductivity detection.Results：The detection limit was 4.95 ng,and the linear range of determination was 9.9-69.3 mg·L-1（r=0.9992）.The recovery rate was 99.2%-101.3%,and the degree of precision was 0.37%,with precision test of RSD 0.82%.Conclusion：This proposed method is simple,rapid,sensitive,accurate and repeatable.

关 键 词：离子色谱法 硫酸小诺霉素 硫酸盐 含量测定 质量控制 

分 类 号：R917[医药卫生—药物分析学]