高效液相色谱法测定暴马子皮药材中2个成分的含量  

HPLC determination of two components in Syringae Cortex

作　　者：宋光西[1] 魏锋[1] 马双成[1] 

机构地区：[1]中国食品药品检定研究院,北京100050

出　　处：《药物分析杂志》2012年第10期1858-1860,1869,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金资助项目(编号:30873356;81173506)

摘　　要：目的:建立测定暴马子皮药材中2个成分(紫丁香苷、sinapylaldehyde 4-O-β-D-glucopyranoside)的高效液相色谱分析方法。方法:采用Phenomenex Luna C18(4.6 mm×250 mm,5μm)色谱柱,流动相为甲醇(B)-0.1%醋酸水溶液(A),梯度洗脱,流速1.0 mL·min-1,DAD检测器,检测波长:284 nm;柱温25℃。结果:紫丁香苷、sinapylaldehyde 4-O-β-D-gluco-pyranoside的线性范围分别为0.2～0.8μg(r=0.9995)和0.04～0.16μg(r=0.9993),平均加样回收率(n=6)分别为99.9%(RSD=2.1%)和99.5%(RSD=2.9%)。结论:不同产地暴马子皮药材中所含紫丁香苷、sinapylaldehyde 4-O-β-D-gluco-pyranoside的含量存在显著性差异。本方法操作简便,结果准确,重复性好,可用于中药暴马子皮药材的质量控制。Objective：To develop an HPLC method for the determination of syringin and sinapyl aldehyde 4-O-β-D-glucopyranoside in Syringae Cortex.Methods：The separation was performed on a Phenomenex Luna C18（4.6 mm×25 0 mm,5 μm） column.The mobile phase was composed of methanol and water solvent containing 0.1% acetic acid with gradient elution.The flow rate was 1.0 mL·min-1 and the detection wavelength was 284 nm.The column temperature was set at 25 ℃.Results：The calibration curves were linear in the ranges of 0.2-0.8 μg for syringin（r=0.9995） and 0.04-0.16 μg for sinapyl aldehyde 4-O-β-D-glucopyranoside（r=0.9993）.The average recoveries（n=6） of syringin and sinapyl aldehyde 4-O-β-D-glucopyranoside were 99.9%（RSD=2.1%） and 99.5%（RSD=2.9%）,respectively.Conclusion：The yields of the two components in different origins exhibit significant differences.This method is simple,accurate and repeatable,which can be used for the quality control of Syringae Cortex.

关 键 词：暴马子皮 化学成分 紫丁香苷 sinapylaldehyde4-O-β-D-glucopyranoside 不同产地含量差异 中药成分分析 综合开发利用 

分 类 号：R917[医药卫生—药物分析学]