基因枪介导法获得转BtCry1Ac基因抗虫玉米植株的研究  

Genetic Transformation of BtCry1Ac into Maize by Particle Bombardment

作　　者：铁双贵[1,2] 柏松[1,3] 岳润清[1,2] 齐建双[1,2] 王延召[1,2] 孙静[1,3] 陈小洁[1,4] 田保明[3] 

机构地区：[1]河南省农业科学院粮食作物研究所,郑州450002 [2]河南省玉米生物学重点实验室,郑州450002 [3]郑州大学生物工程系,郑州450001 [4]河南师范大学生命科学学院,河南新乡453007

出　　处：《玉米科学》2012年第5期54-58,共5页Journal of Maize Sciences

摘　　要：利用基因枪介导法将含有Cry1Ac基因的质粒转入玉米愈伤组织,获得转Bt基因玉米植株,对所获得的转基因植株进行PCR分析和金标免疫试纸鉴定。结果表明,部分转化植株呈阳性,说明目的基因已经整合到玉米基因组中。将转基因植株自交获得T1代种子,对T1代植株进行田间抗虫鉴定,结果表明,与对照相比转基因玉米植株的抗虫活性明显强于非转基因植株。CrylA c gene was introduced into immature embryo callus using particle bombardment. After selection, transgenic plants were regenerated. Some of them were positive by PCR and immune test paper analysis, it was demonstrated that the CrylAc gene had been intergraded Bioassay results showed that T1 generation transgenic plants into plants genome and expressed in transgenic plants. had high resistance to the Asian corn borer.

关 键 词：玉米 转基因 CRY1AC基因 抗虫性 

分 类 号：S513.035.3[农业科学—作物学]