HPLC-MS测定大鼠血浆中青蒿素浓度及其应用  

Determination of artemisinin in rat plasma with HPLC-MS and its application

作　　者：傅川[1] 余继英[2] 邹静[2] 何林[2] 董芸[3] 黄园[1] 

机构地区：[1]四川大学华西药学院,四川成都610041 [2]四川省医学科学院 四川人民医院药剂科,四川成都610072 [3]四川省医学科学院 四川人民医院急诊科,四川成都610072

出　　处：《中国中药杂志》2012年第19期2964-2967,共4页China Journal of Chinese Materia Medica

摘　　要：目的:建立青蒿素大鼠体内药物浓度的分析方法。方法:采用高效液相色谱-质谱联用技术,选用艾司唑仑为内标,样品与内标使用甲基叔丁基醚提取,并分别于m/z305,296进行测定。结果:在5～500μg.L-1,青蒿素峰面积与内标峰面积比值与浓度有良好的线性关系,最低定量质量浓度为5μg.L-1。结论:方法学证明该法能够满足青蒿素大鼠体内血药浓度的测定,可用于动物体内药物动力学的研究需求。Objective： To develop a method for determining artemisinin in rat plasma in vivo. Method： HPLC^MS was adopt- ed. Estazolam was selected as an internal standard （ I. S. ）. The sample and I. S. were extracted using methyl tertbutyl ether and meas- ured at m/z of 305 and 296, respectively. Result ： Within the linear range of 5-500 μg · L-1, the ratio of artemisinin＇s peak area and I.S. peak area and the concentration showed good linearity, thus the minimum concentration was set to be 5 μg · L-1. Conclusion： The methodology proved that the method is so suitable for determining the drug concentration in rat blood that it can be used for stud- ying pharmacokinetics in animals.

关 键 词：青蒿素 高效液相色谱-质谱联用 药物动力学 

分 类 号：R284[医药卫生—中药学]