不同产地野菊花中3种有机酸的含量测定  

Content Determination of Chlorogenic Acid,3,4-ODicaffeoylquinic Acid,3,5-O-Dicaffeoylquinic Acid in Chrysanthemi Indici Flos of Different Areas

作　　者：王丽果[1] 刘伟[1] 吴明侠[1] 

机构地区：[1]河南中医学院,郑州450008

出　　处：《中国实验方剂学杂志》2012年第20期75-78,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南中医学院博士科研基金(BSJJ2009-11);河南省高校青年骨干教师资助计划项目

摘　　要：目的:建立不同产地野菊花中绿原酸、3,4-二咖啡酰奎尼酸、3,5-二咖啡酰奎尼酸的含量测定。方法:采用HPLC方法,色谱柱为Diamonsil(4.6 mm×250 mm,5μm),甲醇-0.1%磷酸溶液做流动相进行梯度洗脱,检测波长326 nm,柱温25℃,流速0.8 mL.min-1。结果:绿原酸、3,4-二咖啡酰奎尼酸和3,5-二咖啡酰奎尼酸的线性范围分别为1.00～20.00,4.64～92.80,2.233～44.66 mg.L-1;加样回收率分别为98.97%,99.14%;99.39%,RSD分别为1.81%,1.66%,1.36%(n=6)。结论:方法操作简便,分析速度快,结果准确,重复性好,可用于野菊花中绿原酸、3,4-二咖啡酰奎尼酸、3,5-二咖啡酰奎尼酸的含量测定。Objective： To determine the content of chlorogenic acid, 3, 4-O-dicaffeoylquinic acid, 3, 5-O-dieaffeoylquinic acid in Chrysanthemi Indici Flos of different areas. Method： HPLC was used. The chromatographic separation was performed on a Diamonsil CIs （4.6 mm ~ 250 mm, 5 p^m） column eluted with gradient mobile phase of methanol-O. 1% Ha PO4, the detection wavelength was set at 326 nm and column temperature was set at 25 ~C. Result： Nice linear relation between the area and content when the amount was within 1.00-20. 00, 4.64-92. 80, 2. 233-44.66 mg ~ L-1 The recoveries were 98.97% , 99. 14% and 99.39% respectively, RSD was 1.81% , 1.66% and 1.36% （n =6） respectively. Coneluslon： The method is accurate, reproducible and suitable to determine these active components in Chrysanthemi Indici Flos. It can be used for quality control of Chrysanthemi Indici Flos.

关 键 词：高效液相色谱法 含量测定 绿原酸 3 4-二咖啡酰奎尼酸 3 5-二咖啡酰奎尼酸 

分 类 号：R283.6[医药卫生—中药学]