HPLC测定止痒乳膏中间体中黄芩苷的含量  

Determination of Baicalin in Relieving Itching Cream Intermediate Products by HPLC

作　　者：朱红[1] 周旭[1] 张诗龙[1] 周燕萍[1] 刘峰群[1] 

机构地区：[1]中国人民解放军第302医院药学部,北京100039

出　　处：《中国实验方剂学杂志》2012年第20期106-108,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:采用高效液相色谱法测定止痒乳膏中间体中黄芩苷的含量。方法:采用Eclipse Plus C18色谱柱,以甲醇-0.37%磷酸为流动相,梯度洗脱,检测波长280 nm,流速1.0 mL.min-1。结果:黄芩苷在0.0792～0.792μg线性关系良好(r2=0.999 9),回收率98.87%,RSD 1.14%(n=6);监测3批止痒乳膏中间体表明:黄芩水提物中黄芩苷的含量分别为5.75,6.08,5.94 g.L-1。结论:方法稳定、灵敏、准确,能够有效的监控上述中间体的质量,可作为该制剂生产过程中质量控制。Objective： To establish a high performance liquid chromatographic method （HPLC） for determination of baicalin in relieving itching cream intermediate products. Method： Agilent Eclipse Plus Cls column was used to separate the analyte with a mobile phase consisting of methyl alcohol and 0. 37% phosphoric acid at a flow rate of 1 mL min- 1 The detection wavelength was set at 280 nm. Result： Baicalin showed good linearity （r2 =0. 999 9） in the range of the tested concentration （0. 0792-0. 792 pLg） ; the average recovery of the method was 98.87% with RSD 1.14% （n = 6）. This method was used to successfully determine 3 batches relieving itching cream intermediate products, the content of baicalin in aqueous extract of Scutellaria Baicalensis was 5.75, 6.08, 5.94 g · L-1. Conclusion： This is a more stable, sensitive and precise method for monitoring relieving itching cream intermediate products, which could also be used as the quality control method for the process of the relieving itching cream.

关 键 词：止痒乳膏 黄芩苷 高效液相色谱法 中间体 

分 类 号：R283.6[医药卫生—中药学]