异钩藤碱与天麻素合用对异钩藤碱在大鼠肝脏分布的影响  

Distribution of Isorhynchophyline in Liver Tissures before and after Compatibility with Gastrodin and Isorhynchophylline in Rats

作　　者：陈志远[1] 王兴[1] 李晓倩[1] 王新勇[1] 张卫国[1] 

机构地区：[1]西南交通大学生命科学与工程学院,成都610031

出　　处：《中国实验方剂学杂志》2012年第20期193-196,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30801547);中央高校基本科研业务费专项资金项目(SWJTU11ZT26;SWJTU09ZT29)

摘　　要：目的:观察天麻素与异钩藤碱合用前后异钩藤碱在SD大鼠肝脏的分布变化。方法:采用高效液相色谱法测定异钩藤碱与天麻素合用前后异钩藤碱在SD大鼠肝脏中的含量。色谱条件:WatersTM色谱柱(4.6 mm×250 mm,RP 18.5μm);流动相甲醇-水(70∶30),pH 8.0,流速0.8 mL.min-1,柱温30℃,检测波长254 nm,进样量20μL。结果:异钩藤碱对照品在肝脏组织中在0.16～4.0 mg.L-1具有较好的线性关系;异钩藤碱与天麻素配伍前后在肝脏中的分布无显著性差异。结论:天麻素有一定的降低异钩藤碱在肝脏分布的作用。Objective： To observation the distribution of isorhynchophyline in liver tissues before and after compatibility with gastrodin and isorhynchophylline in rats. Method： HPLC was employed to determinate the isorhynchophyline in liver tissuesin rats before and after compatibility with gastrodia and unicaria; analyticalcolumn was WatersTM （4.6 mm x250 ram, RP 18.5 pm） ; the mobile phase consisted of methanol-water （70： 30） , pH 8.0, fluid rate was 0. 8 mL rain-1 ; the column temperature was 30 C , the detector wave length was set at 254 nm, the sample amount is 20 p.L. Reslut： Isorhynchophyline in liver and kidney tissues was linear in the range of 0. 16-4.0 mg L-1. There were no significant differences in liver tissues of SD rats before and after compatibility. Conclusion： Gastrodin has a certain effects reduction in the liver tissues distribution.

关 键 词：钩藤 天麻 天麻素 异钩藤碱 

分 类 号：R285.5[医药卫生—中药学]