复方阿胶浆对H22肝癌荷瘤小鼠5-FU化疗的增效减毒作用  

Effects of Fufang Ejiao Jiang Enhances Efficacy and Reduces Toxicity of 5-FU in Hepatoma H22-bearing Mice

作　　者：栗敏[1] 马洪宇 沈继朵[3] 栗俞程[3] 

机构地区：[1]郑州人民医院,郑州450003 [2]郑州市中医院,郑州450007 [3]河南中医学院,郑州450008

出　　处：《中国实验方剂学杂志》2012年第20期216-219,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南中医学院博士科研基金项目(BSJJ2010-24)

摘　　要：目的:探讨复方阿胶浆对5-氟尿嘧啶(5-FU)抗小鼠H22肝癌的增效减毒作用。方法:SPF级昆明种小鼠接种瘤细胞制备H22肝癌小鼠模型,将肝癌小鼠随机分为荷瘤模型组、5-FU对照组(20 mg.kg-1,ip)、5-FU联用复方阿胶浆高、中、低剂量组(3,1.5,0.75 g.kg-1,以阿胶生药量计)。另设正常对照组(n=10)。连续给药7 d后处死动物,检测外周血白细胞计数,计算瘤重、抑瘤率、脾脏指数和胸腺指数。采用淋巴细胞转化实验检测外周血淋巴细胞转化率;采用鸡红细胞吞噬实验检测腹腔巨噬细胞的吞噬功能。结果:与5-FU对照组相比,高剂量复方阿胶浆联合治疗组小鼠瘤质量明显减轻(P<0.05),脾脏指数、胸腺指数以及外周血白细胞计数均显著提高(P<0.05)。5-FU化疗使荷瘤小鼠淋巴细胞转化率、腹腔巨噬细胞吞噬率和吞噬指数较荷瘤模型组均显著降低(P<0.01)。复方阿胶浆联合治疗组小鼠淋巴细胞转化率、腹腔巨噬细胞吞噬率和吞噬指数均显著高于5-FU对照组(P<0.05),其中高剂量组分别达(35.9±9.2)%,(41.9±6.3)%,(0.74±0.17)。结论:复方阿胶浆对5-FU抗小鼠H22肝癌具有明显的化疗增效减毒作用。Objective： To study the effects of Fufang Ejiao Jiang （FFEJJ） against 5-fluorouracil （5-FU） chemotherapy in hepatoma H22-hearing mice. Method： The SPF-Kunming mice were used to establish mouse model of hepatoma H22. The mice were randomly divided into 5 groups： tumor model group, 5-FU group （20 mg ~ kg-1, ip）, 5-FU combined FFEJJ group. FFEJJ were orally administrated at a daily dose of 3, 1.5, O. 75 g .kg-~ , respectively corresponding to high, middle and low dose. The normal control group was set up and each group had 10 mice. After continuous administration for 7 d, the peripheral white blood cell count （WBC） , tumor weight, inhibition rate, spleen index and thymus index were measured. The peripheral blood lymphocyte transformation rate and the phagocytotic activity of peritoneal macrophages were detected. Result： The high-dose FFEJJ significantly reduced the tumor weight, elevated the spleen index, thymus index and WBC counts compared with 5-FU group （P 〈 0.05）. 5-FU decreased the lymphocyte transformation rate and peritoneal macrophage phagocytosis rate and the phagocytic index （P 〈0.01 ）. These index in FFEJJ group were significantly higher than that of 5-FU group （P 〈0.05）, and reached to （35.9 ±9.2）% , （41.9±6.3）% , and （0.74±0. 17） in high dose FFEJJ group respectively. Conelusion： The results suggest that FFEJJ could enhance efficacy and reduce toxicity in hepatoma treatment as a supplement of 5-FU.

关 键 词：复方阿胶浆 肝癌H22小鼠 5-氟尿嘧啶 免疫功能 抑瘤率 

分 类 号：R285.5[医药卫生—中药学]