半夏总生物碱对D-半乳糖所致衰老小鼠学习记忆障碍的改善作用  

Effects of Total Alkaloids from Pinellia ternate on Learning and Memory in Aging Mice Induced by D-Galactose

作　　者：唐瑛[1] 雷呈祥[1] 段凯[2] 王晓昆[3] 杨李[3] 朱以良[3] 

机构地区：[1]海军医学研究所,上海200433 [2]湖北中医药大学,武汉430070 [3]广州军区武汉总医院,武汉430070

出　　处：《中国实验方剂学杂志》2012年第20期224-227,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:研究半夏总生物碱(TAPT)对D-半乳糖诱导的衰老小鼠学习记忆的影响并初步探讨其作用机制。方法:昆明种小鼠100只,随机分为5组,即正常对照组,模型组,TAPT低、中、高剂量组(7.5,15,30 mg.kg-1),每组20只。模型组和TAPT组给予120 mg.kg-1的D-半乳糖注射液(sc,每天1次,连续8周),建立学习记忆障碍的衰老小鼠模型,对照组给予等量生理盐水;同时,TAPT各组给予对应浓度的TAPT药液(ig,每天1次,连续8周),对照组及模型组给予等量蒸馏水。采用跳台法和Y迷宫评价法测试TAPT对模型小鼠学习记忆障碍的改善作用,采用比色法测定小鼠脑组织中丙二醛(MDA)的含量,超氧化物歧化酶(SOD)和乙酰胆碱酯酶(AChE)的活性。结果:TAPT低、中、高剂量组小鼠Y迷宫训练时达标所需总次数减少,分别为(21.9±2.9),(20.8±3.2),(19.7±2.8)次;Y迷宫记忆测试时错误次数也明显减少,分别为(3.8±1.4),(3.2±1.7),(1.7±1.1)次;模型组小鼠脑组织SOD活性明显降低(28.98±13.08)U.mg-1,MDA及AChE含量明显增加(2.78±0.21)nmol.mg-1,(1.05±0.16)U.mg-1;经TAPT干预后,各剂量组小鼠脑组织中SOD活性增高(P<0.01),MDA含量明显降低(P<0.01);中、高剂量组AChE含量显著减少(P<0.01,P<0.05)。结论:应用TAPT干预D-半乳糖诱导的衰老小鼠,可明显改善其学习和记忆功能,这可能与其具有抗氧化作用及抑制AChE的活性有关。Objective： To study the effects of total alkaloids from Pinellia ternate （TAPT） on learning and memory ability in aging mice induced by D-galactose and its preliminary mechanism. Method： One hundred mice were randomly assigned into control group, model group, TAPT low, middle and high dose group （7.5, 15, 30 mg kg-1） , 20 mice in each group. The TAPT and the model groups were given 120 mg kg-lD-galactose （sc, for 8 weeks） to induce aging-mouse model, and the control group was given the same volume of saline water; meanwhile, the TAPT groups were given appropriate liquid （ig, for 8 weeks）, while the control and the model groups were given the same volume of distilled water. The step-down test and the Y-maze test were adopted to reflect the learning and memory of mice ; and the colorimetric method was used to determine the cellular content of malondialdehyde （ MDA）, the enzyme activity of superoxide dismutase （SO13） and acetylcholinesterase （ACHE）. Result： Compared to the model group, the TAPT treatment brought mice to shorten the time to reach the final in the Y-maze training at （21.9 ± 2.9）, （20. 8 ± 3.2）, （19.7 ± 2.8） times respectively, and also reduce the mistakes in memory test at （3.8±1.4）, （3.2 ±1.7）, （1.7 ±1.1） times respectively. SOD activity in brain tissues of aging model mice reduced evidently at （28.98 ± 13.08） U mg-1, while MDA and AChE contents increased evidently at （2.78 ± 0.21） nmol .mg-1, （1.05 ±0.16） U mg-1, while TAPT treatment could evidently decrease MDA （P 〈 0.01 ） and AChE contents （P 〈 0.01 ）, and increase SOD activity （P 〈 0.01 ）. Conclusion： TAPT treatment could help aging model mice induced by D-galactose evidently to enhance their learning and memory, which might be related to its antioxidation and its inhibitory activity to ACHE.

关 键 词：半夏总生物碱 D-半乳糖 衰老小鼠 学习记忆 

分 类 号：R285.5[医药卫生—中药学]