溃疡平对阿司匹林诱导的复发胃溃疡大鼠胃黏膜组织中VEGF和Flt-1 mRNA表达的影响  

Effect of Kuiyangping on Expression of VEGF and Flt-1 mRNA in Gastric Mucosa of Rats with Aspirin-induced Gastric Ulcer Recurrence

作　　者：赵堃[1] 赵红晔[1] 牛淑冬[1] 王滨[1] 

机构地区：[1]齐齐哈尔医学院生理教研室,黑龙江齐齐哈尔161006

出　　处：《中国实验方剂学杂志》2012年第20期267-269,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：黑龙江省教育厅科学技术研究项目(12511629)

摘　　要：目的:以非甾体类抗炎药物阿司匹林所致胃溃疡复发大鼠为模型,探讨溃疡平抗胃溃疡复发的机制。方法:以Okeba改良法复制大鼠实验性胃溃疡,ig阿司匹林制作复发模型,采用RT-PCR法观察溃疡平5.6 g.kg-1,连续ig 14 d对胃溃疡组织血管内皮生长因子(vascular endothelial growth factor,VEGF)mRNA表达的影响。结果:溃疡平组VEGF mRNA和VEGF受体(Flt-1)mRNA的表达较复发组显著增高(P<0.01)。结论:溃疡平可提高胃黏膜中VEGF和Flt-1 mRNA的表达,从而增加黏膜中微血管通透性,将胃内有害物质稀释,并且刺激黏膜中腺体和血管的生成,达到保护胃黏膜的作用。这可能是溃疡平促进胃溃疡愈合,减少其复发的重要机制之一。Objective： To investigate the anti-ulcer recurrence mechanism of Kuiyangping through establishing experimental chronic ulcer model. Method： Okeba modified method was used to establish gastric ulcer model in rats by administering intragastrically with aspirin once a day. Kuiyangping of 5.60 g kg- 1 was administrated for 14 days. The RT-PCR method was used to detect the expressions of vascular endothelial growth factor （VEGF） and its receptor Flt-1 mRNA in the gastric mucosa surrounding ulcer. Result： Kuiyangping could increase the expression of VEGF and Flt-1 mRNA in the gastric mucosa surrounding ulcer （compared with recurrence group, P 〈0.01 ）. Conclusion： Kuiyangping could decrease the gastric ulcer recurrence induced by aspirin. The effective mechanisms might be related to the enhancement of the expression of VEGF and Fh-1 mRNA, the increase in microvaseular permeability, and the promotion of vasculogenesis in the gastric mucosa surrounding ulcer.

关 键 词：溃疡平 胃溃疡复发 血管内皮生长因子MRNA 实时PCR 

分 类 号：R285.5[医药卫生—中药学]