受体来源转染pBLAST2-hHGF质粒肝卵圆细胞对大鼠移植肝肝功能的影响  

Improving liver function on recipient rats by recipient derived Hepatic oval cells(pBLAST2-hHGF Transfected) graft implanting

作　　者：李铸[1] 陈娟[1] 李学华[1] 刘志恒[1] 刘桂杰[1] 张学利[1] 高延超[1] 孙会东[1] 纪丕友[1] 张金良[1] 李立[2] 

机构地区：[1]山东省聊城市人民医院,山东聊城252000 [2]云南省昆明市第一人民医院,云南昆明650011

出　　处：《中国现代医学杂志》2012年第23期51-57,共7页China Journal of Modern Medicine

摘　　要：目的观察受体来源转染pBLAST2-hHGF质粒肝卵圆细胞(HOC)对受体大鼠术后肝功能的影响。方法二袖套法复制大鼠原位肝移植模型。实验分对照组(A组)、HOC移植组(B组)、pBLAST2-hHGF/HOC移植组(C组),每组均设受体大鼠60只,A组仅行原位肝移植术,B组术中供肝种植HOC悬液,C组术中供肝种植pBLAST2-hHGF/HOC悬液。动态观察各组大鼠术后的中位生存时间(MST)、肝功能(ALT、DBil、GGT、ALP、XCHE和ALB)。结果 A组术后MST明显短于B组和C组,而B组又短于C组;B组和C组大鼠术后肝细胞受损情况弱于A组(P<0.05),合成功能和排泄功能好于A组(P<0.05),上述肝功能指标,C组要好于B组(P<0.05)。结论使用受体来源pBLAST2-hHGF/HOC在移植肝脏内进行种植,可以有效改善移植肝脏的肝功能,并且明显延长受体的术后中位生存时间。【Objective】 To observe the liver function on recipient rats after liver transplantation and pBLAST2-hHGF/HOC（recipient origin） liver graft implanting.【Methods】 Rats orthotopic liver transplantation models were established by using Kamada two-cuff technique.Recipients were divided into control group（group A）,HOC implanting group（group B） and pBLAST2-hHGF/HOC group（group C）,each group includ 60 recipients.Recipients in group A only establish orthotopic liver transplantation models,while recipients in group B adding a procedure of liver graft HOC implanting,recipients in group C adding a procedure of liver graft pBLAST2-hHGF/HOC implanting during liver transplantation.The median survival time（MST） and liver functions（ALT,DBil,GGT,ALP,XCHE,ALB） in each group after liver transplantation were observed.【Results】 The MST in group B and group C were longer than those in group A（P 0.05）,while MST in group B was shorter than that in group C（P 0.05）.Degree of hepatocytes injury in group B and group C were more slightly than those in group A（P 0.05）,and liver synthetic function and liver excretion function in group B and group C were much better than those in group A（P 0.05）.By the same time,indexes of liver function mentioned above in group C were better than those in group B（P 0.05）.【Conclusions】 The implantation of pBLAST2-hHGF/HOC（recipient origin） to liver graft could effectively improve liver function,significantly prolong MST in recipients after liver transplantation.

关 键 词：肝移植 大鼠 肝卵圆细胞 肝功能 

分 类 号：R657.3[医药卫生—外科学]