亚急性硒中毒对小鼠巨噬细胞与抗体形成细胞的影响  

Effect of subacute selenite poisoning on macrophage and antibody producing cells in mice

作　　者：管延杰[1] 张蕾[1] 陈爽[1] 侯振中[1] 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030

出　　处：《中国兽医杂志》2012年第9期13-15,共3页Chinese Journal of Veterinary Medicine

摘　　要：为探讨亚硒酸钠染毒对小鼠免疫功能的影响,将试验动物分为阴性对照组(0.00mg/kg体重.d)、亚硒酸钠低剂量组(0.14mg/kg体重.d)、中剂量组(0.54mg/kg体重.d)和高剂量组(2.16mg/kg体重.d)。连续灌胃30d后处死小鼠,分别用巨噬细胞吞噬鸡红细胞试验与溶血空斑试验检测小鼠腹腔巨噬细胞的吞噬功能与抗体形成细胞的数量。结果显示,亚硒酸钠染毒使小鼠巨噬细胞的吞噬功能与抗体形成细胞的数量显著降低,且与染毒剂量具有相关性。表明亚硒酸钠染毒可抑制小鼠非特异性免疫功能,呈现明显剂量效应关系。To investigate the effect of sodium selenite poisoning on the immune functions in mice. The experimental animals were divided into control group （0.00 mg/kg . d）, low dose（0.14 mg/kg. d）, middle dose group （0.54 mg/kg . d） and high dose（2.16 mg/kg. d）. Sodium selenite groups mice were killed after continuous intragastric feeding for 30 days. Phagocytosis of chicken red blood cells by macrophage and hemolytic plaque assay were used to examine the phagocytic function of mouse peritoneal macrophages and numbers of antibody producing cells. Sodium selenite poisoning significantly resuced the phagocytic function of mouse peritoneal macrophages and the number of antibody producing cells. It has dose-dependent correlation. Sodium selenite poisoning inhibits the function of nonspecific immunity in mice, which presents the apparent dose effect relationship.

关 键 词：亚硒酸钠 巨噬细胞 溶血空斑 非特异性免疫 

分 类 号：S859.8[农业科学—临床兽医学]