云南鸭疫里默氏杆菌外膜蛋白A及16S rRNA序列分析  

The Sequence Analysis of OmpAand 16S rRNA Genes of Riemerella anatipestifer Isolated Strains in Yunnan Province

作　　者：李富祥 王传禹 常志顺 杨斌 李华春 

机构地区：[1]云南省畜牧兽医科学院,云南昆明650224

出　　处：《中国畜牧兽医》2012年第10期61-65,共5页China Animal Husbandry & Veterinary Medicine

基　　金：云南省自然科学基金(2010ZC228);科研院所技术开发研究专项(2007CF009)

摘　　要：为了探讨鸭疫里默氏杆菌(Riemerella anatipestifer,RA)云南流行株的外膜蛋白A(OmpA)的基因序列差异及其与16SrRNA序列的相关性,PCR扩增18株云南流行株鸭疫里默氏杆菌OmpA基因及16SrRNA核苷酸序列,分别构建其系统进化树,分析其系统进化关系。结果表明,18株鸭疫里默氏杆菌OmpA基因分为2个群,其同源性分别为86%～99.2%和92.6%～100%。18株鸭疫里默氏杆菌16SrRNA基因同属1个群,同源性高达96.1%～100%。RA-1、RA-2、RA-11和RA-39 4株分离株的OmpA基因位于进化树的同一个亚群,其16SrRNA基因也位于进化树的同一亚群,两者呈现出明显的相关关系,其他14株分离株的OmpA基因系统进化树与16SrRNA基因系统进化树无明显的相关关系。To analysis the OmpA and 16S rRNA genes of Riemerella anatipestifer isolated strains in Yunnan province and explore the correlationship between OmpA and 16S rRNA genes on phylogenetic trees, OmpA and 16S rRNA genes were am- plified respectively by PCR to construct the phylogenetic trees. Results analysis showed that the OmpA genes of 18 Yunnan i- solated RA strains were situated in two groups and their homology were 86 % to 99.2% and 92.6 % to 100 % respectively, the 16S rRNA genes of 18 Yunnan isolated RA strains were situated in one group and their homology was 96.1% to 100%. It＇s worth noting that the RA-1 ,RA-2 ,RA-1 and RA-39 isolated strains belonged to the same subgroup on both the OmpA phylo- genetic tree and 16S rRNA phylogenetic tree with the higher homology 97.3% to 99.2% and 99.6% to 100% respectively, the two phylogenetic trees showed a significant correlation within RA-1, RA-2, RA-1 and RA-39 isolated strains and showed no cor- relation within the other 14 isolated strains.

关 键 词：鸭疫里默氏杆菌 外膜蛋白A基因 16S RRNA基因 序列分析 

分 类 号：S855.12[农业科学—临床兽医学]