微孔滤膜碱洗脱法检测DNA损伤  

Detection of DNA Damage by Microfilter Alkaline Elution

作　　者：居学海[1] 张琪[2] 戴乾圜[1] 

机构地区：[1]北京大学环境科学中心,北京100871 [2]北京工业大学癌化学与生物工程中心,北京100022

出　　处：《北京工业大学学报》2000年第2期49-53,共5页Journal of Beijing University of Technology

摘　　要：碱洗脱法作为检测哺乳动物多种类型ＤＮＡ损伤的简便而又经济的方法在致突、致癌及抗癌的研究领域得到了广泛的应用．对该法的原理、操作技术及进展进行了综述．The alkaline elution methods were simple and econondc methods to detect sevens types of DNA damage in mammalian cells. They were widely used in the field of Potential potential, mutagens, carcinogens and anticancer agents. The essential concepts and theory of the technique were discussed and applications of technique to various types of DNA damages were reviewed.

关 键 词：DNA损伤 微孔滤膜 检测 碱洗脱法 单链断裂 

分 类 号：Q343.245[生物学—遗传学] Q523.03