以子房为外植体的‘金娃娃’萱草组织培养技术的研究  

Study on Tissue Culture Technique in Hemerocallis hybridus'Stella D'oro'Using Ovary as Explants

作　　者：杨丽莉[1] 王德平[2] 张晓 张彦琴[1] 李洁 

机构地区：[1]山西省农业科学院旱地农业研究中心,太原030006 [2]中国生物技术发展中心,北京100036 [3]山西省襄垣县第二中学,山西襄垣046200

出　　处：《中国农学通报》2012年第25期184-190,共7页Chinese Agricultural Science Bulletin

基　　金：山西省科技攻关项目"新型耐旱缀花地被植物大花萱草的组培快繁技术研究"(041012-3)

摘　　要：为提高萱草的繁殖效率,以‘金娃娃’萱草花蕾为试验材料,采用以子房为外植体的初代培养方法,对其组织培养技术体系进行了研究。结果表明:采用高强度灭菌花蕾,无菌剥离子房的技术方法,初代培养实现了零污染、零死亡;以子房为外植体的愈伤组织诱导分化以6-BA2mg/L+2,4-D1mg/L+NAA0.4mg/L效果最佳。通过对芽增殖培养基中基本培养基、激素配比和高有机物的研究发现,基本培养基B5好于MS,激素以6-BA2mg/L+NAA0.3mg/L效果最好,繁殖系数达到7.2;添加L-脯氨酸和水解络蛋白对繁殖系数影响不大,但是,可以有效改善试管苗的生长状态;试管苗的生根以1/2MS+0.4mg/L NAA+20g/L蔗糖效果最好;试管苗在粗河沙、蛭石+泥炭土(3:1)和泥炭土3种介质中的成活率均可达到90%。In order to improve the efficiency of propagation of Hemeroeallis hybridus,this study researched the tissue culture system with ovary as explant in primary culture method;use flower bud of＇Stella D＇oro＇as the test material.The result showed that：the first generation cultivated achieved zero pollution and zero perishing by adopting high density sterilizing buds and the technique of peeling ovary in aseptic,and medium supplemented with 6-BA 2 mg/L ＋ 2,4-D 1 mg/L ＋ NAA 0.4 mg/L was the best one for ovary explant callus induction and differentiation.Through studying the basic culture medium,hormone ratio and the highly organic substances of the bud multiplications,it is found that：the basic culture medium B5 was better than MS.The hormone supplemented took effect best with 6-BA 2 mg/L＋NAA 0.3 mg/L,the propagation coefficient reached 7.2.Adding L-proline and hydrolysis of complex protein on propagation coefficient had little effect on,but,they can effectively improve the growth state of plantlet.The medium supplemented with 1/2MS ＋ 0.4 mg/L NAA ＋ 20 g/L sucrose was the best for rooting of plantlet.The survival rates of plantlets reached 90% in three conditions：river sand,peaty soil,and 3：1 of vermiculite＋peaty soil.

关 键 词：子房 '金娃娃’萱草 外植体 组织培养 

分 类 号：Q813.1[生物学—生物工程]