不同产地人参中4种脱氢酶活力比较  

Comparison of Activity of Four Dehydrogenases in Ginseng from Different Origins

作　　者：杨菲[1] 赵雨[1] 王思明[1] 刘美辰[1] 李晓华[1] 

机构地区：[1]长春中医药大学,长春130117

出　　处：《中国农学通报》2012年第25期224-228,共5页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金资助项目"人参蛋白抗衰老机制研究"(30873371);国家科技支撑计划资助项目"长白山特种药材技术标准及关键技术研究与开发"(2007BAI38B02)

摘　　要：为了给人参的培育和优选提供理论依据,采用中性缓冲液提取粗酶液,应用分光光度法对15个不同产地的人参中苹果酸脱氢酶(malate dehydrogenase,MDH)、乳酸脱氢酶(lactate dehydrogenase,LDH)、乙醇脱氢酶(alcohol dehydrogenase,ADH)、葡萄糖-6-磷酸脱氢酶(glucose-6-phosphate dehy drogenase,G6PDH)4种脱氢酶活力进行比较。运用SPSS13.0软件对15批样品进行系统聚类分析。结果表明不同产地人参脱氢酶活力差别明显,同一产地4种脱氢酶活力趋势基本相同。其中安图县万宝镇的人参样品的MDH、LDH、G6PDH3种酶活力均是最高值,分别为124.58U/(g·FW)、129.88U/(g·FW)、109.84U/(g·FW);黑龙江2个产地的4种酶活力普遍比较低。聚类分析的结果将样品分为4类。MDH、LDH、ADH、G6PDH的活力可以作为人参培育和优选提供理论依据。The aim was to provide theoretical basis for the cultivation and optimization of ginseng.Adopt neutral buffer solution to extract the enzyme solution of Radix Ginseng.The activities of malate dehydrogenase（MDH）,lactate dehydrogenase（LDH）,alcohol dehydrogenase（ADH） and glucose-6-phosphate dehydrogenase（G6PDH） were detected by spectrophotometry,and compared.The clustering analysis was performed using the software SPSS 13.0 to system for 15 batch sample.There were obvious differences of the activities dehydrogenase of ginseng from different origin.The activities of four dehydrogenases from the same origin were basically same.In Antu County Wanbao Town,MDH,LDH and G6PDH had the highest activities,124.58 U/（g·FW）,129.88 U/（g·FW） and 109.84 U/（g·FW） respectively.The four kinds of enzymes activity of two origins in Heilongjiang Province were generally low.The sample was divided into four categories.The activities of MDH,LDH,ADH and G6PDH could provide theoretical basis for the cultivation and optimization of ginseng.

关 键 词：人参 脱氢酶 苹果酸脱氢酶 乳酸脱氢酶 乙醇脱氢酶 葡萄糖-6-磷酸脱氢酶 

分 类 号：R284.1[医药卫生—中药学]