真菌菌丝黑色素提取条件的研究  

Study of Extracting Condition of Fungal Melanin

作　　者：杨一艳[1] 李明锐[1] 包龙丽[1] 何永美[1] 

机构地区：[1]云南农业大学资源与环境学院,云南昆明650201

出　　处：《安徽农业科学》2012年第29期14161-14162,共2页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(41101486);云南省教育厅重点科研基金项目(09Z0037)

摘　　要：[目的]为了更好地提取真菌黑色素。[方法]比较了菌丝粒径(30、60、100和140目)和3种高温提取条件(高压灭菌锅内121℃20 min、沸水浴2 h和沸水浴5 h)提取真菌黑色素的效果。[结果]菌丝粒径对黑色素提取效果影响不显著。沸水浴5 h提取不同粒径菌丝黑色素的含量在0.01水平显著高于其余2个高温提取条件。沸水浴5 h提取30目菌丝黑色素含量的RSD为0.5%～3.0%,黑色素加标回收率为86.0%～123.0%。[结论]1 mol/L NaOH沸水浴5 h提取30目菌丝中的黑色素是较好的提取方法。[Objective] The research aimed to extract the fungal melanin better.[Method] The effects of the particle size（30,60,100 and 140 mesh） of fungal mycelium and 3 kinds of extraction conditions at high temperature（121 ℃ and 20 min in high-pressure sterilization pot,boiling water bath for 2 h and boiling water bath for 5 h） on the extraction of fungal melanin were studied.[Result] No significant influence of the particle size of fungal mycelium on the extraction of fungal melanin was found.The content of fungal melanin was extracted by boiling water bath for 5 h was significantly higher than that by 121 ℃ and 20 min in high-pressure sterilization pot or by boiling water bath for 2 h.RSD and recovery rate of fungal melanin extracted from fungal mycelium of 30 mesh by boiling water bath for 5 h was 0.5%-3.0% and 86.0%-123.0% respectively.[Conclusion] The method was feasible for the extraction of fungal melanin from fungal mycelium of 30 mesh by 1 mol/L NaOH with boiling water bath for 5 h.

关 键 词：真菌黑色素 粒径 温度 时间 

分 类 号：S181.3[农业科学—农业基础科学]