不同品种紫花苜蓿愈伤组织及分化体系的建立  

Establishment of the Callus and Differentiation System of Different Alfalfa Varieties

作　　者：张二芹[1] 马强[2] 王云[1] 高宛莉[1] 郭亚君[1] 

机构地区：[1]南阳师范学院生命科学与技术学院,河南南阳473061 [2]河南省农业科学院畜牧兽医研究所,河南郑州45002

出　　处：《安徽农业科学》2012年第29期14172-14173,共2页Journal of Anhui Agricultural Sciences

基　　金：河南省教育厅自然科学研究计划项目(2009B230010)

摘　　要：[目的]建立不同品种紫花苜蓿的愈伤组织及分化体系。[方法]以4个品种的无菌苗子叶为外植体,研究不同的激素浓度及基因型对紫花苜蓿胚性愈伤组织诱导及分化的影响。[结果]4个品种诱导形成的愈伤组织差别不大;最佳胚性愈伤组织诱导培养基为改良SH+2,4-D 2 mg/L+KT 0.05 mg/L;苜蓿王的分化率和出苗率表现较佳;最佳分化培养基为改良SH+KT 0.4 mg/L。[结论]该试验为今后筛选易分化的苜蓿品种和研究"转基因苜蓿疫苗"奠定了基础。[Objective] To establish the callus and differentiation system of different alfalfa varieties.[Method] The cotyledon of four varieties of alfalfa（Medicago sativa L.） were used to study the effect of hormones in different concentrations and genotypes on embryogenic callus and differentiation.[Result] The embryogenic callus rate of four varieties of alfalfa were little difference;the best medium for embryogenic callus induction was the modified SH＋2.0 mg/L 2,4-D＋0.05 mg/L KT;the differentiation and plant development rate of Muxuwang variety was the highest;the best medium for differentiation was the modified SH＋0.4 mg/L KT.[Conclusion] The study laid a foundation for screening easy-differentiation alfalfa variety and researching transgenic alfalfa vaccine.

关 键 词：紫花苜蓿 组织培养 愈伤组织 分化 

分 类 号：S541.1[农业科学—作物学]