L-色氨酸分子印迹传感器敏感膜的制备与性能  

Preparation and Performance of Sensing Films of Molecularly Imprinted Electrochemical Sensor for L-tryptophan

作　　者：徐雯[1] 李晓[1] 张卫英[1] 英晓光[1] 

机构地区：[1]福州大学化学化工学院,福州350108

出　　处：《高等学校化学学报》2012年第10期2199-2204,共6页Chemical Journal of Chinese Universities

基　　金：福建省高等学校新世纪优秀人才支持计划(批准号:HX2006-102);科技部国际合作重点项目(批准号:2009DFA61680);福建省自然科学基金(批准号:2010J01037)资助

摘　　要：以L-色氨酸(L-Trp)为模板分子,邻苯二胺(o-PD)为功能单体,在金电极表面原位合成了分子印迹聚合物敏感膜;通过循环伏安法(CV)、差示脉冲伏安法(DPV)和电化学阻抗谱法(EIS)考察了该电极的性能.DPV测试结果表明,在1×10-8～2×10-7mol/L范围内,峰电流与L-Trp的浓度呈线性关系,检出限为0.3×10-8mol/L;选择识别性实验结果表明,L-Trp印迹敏感膜的印迹因子达到3.72,相对于干扰物的选择因子均大于1,对与L-Trp结构相似的L-酪氨酸(L-Tyr)的选择因子也达到2.30,说明该印迹膜对L-Trp具有良好的选择性;识别过程动力学研究结果表明,印迹膜对L-Trp的识别是一个两步连续发生的过程,即快结合过程和慢吸附过程.Using L-tryptophan（L-Trp） as template molecule and o-phenylenedia mine as functional monomer, the sensitive molecularly imprinted polymer films was prepared on the surface of gold electrode by in-situ polymerization. The performance of the imprinted electrode was characterized by cyclic voltammetry （CV） , differential pulse vohammetry （DPV） and electro-chemical impedance spectroscopy （EIS）. A linear relation- ship between oxidation peak current and concentration of L-Trp was obtained over a range from 1 ×10-8 to 2× 10-7 mol/L with a detection limit of 0.3 × 10-8 moL/L. From the selectivity experiments, the imprinting factor of L-Trp imprinted film was 3.72 and the selectivity factors of L-Trp compared to interferents were all larger than 1. Although a minor interference was generated by L-Tyr for its structure similar to L-Trp, the selectivity factor also reached 2.30. The results indicated that the imprinted polymer films had a good selectivity for L-Trp. The kinetics of recognition revealed a two-step process of the imprinted polymer films adsorbing L-Trp, which contains a fast binding process and a slow adsorption process.

关 键 词：L-色氨酸 分子印迹 电化学聚合 传感器敏感膜 识别过程 

分 类 号：O657.1[理学—分析化学] O646[理学—化学]