大黄酸磁性印迹聚合物的制备及其应用研究  

Preparation and Application of Rhein Magnetic Molecularly Imprinted Polymer

作　　者：刘玉楠[1,2] 张朝晖[1,2,3] 张明磊[1] 陈星[1] 陈红军[1] 聂丽华[3] 

机构地区：[1]吉首大学湖南省林产化工重点实验室,张家界427000 [2]吉首大学化学化工学院,吉首416000 [3]湖南大学化学生物传感与计量国家重点实验室,长沙410082

出　　处：《化学通报》2012年第9期842-847,共6页Chemistry

基　　金：国家自然科学基金项目(21005030);湖南省教育厅重点项目(10A099);十二.五国家科技支撑计划项目(2011BAD10B01);湖南省高校林产资源化学与林化产品开发科技创新团队支持计划资助

摘　　要：以SiO2包覆的磁性粒子为载体,大黄酸为模板分子,苯基和氨基硅烷偶联剂为二元功能单体,正硅酸乙酯为交联剂制备大黄酸磁性印迹聚合物。采用红外光谱和扫描电镜对该印迹聚合物进行表征。结果表明,该印迹聚合物颗粒分散均匀,呈立方体结构,印迹壳层厚度约75～100nm。结合磁固相萃取技术和高效液相色谱技术对大黄蒽醌类组分进行富集分离,实现猕猴桃根提取液中大黄酸、大黄素、大黄酚和大黄素甲醚等组分的同时分离、富集和检测。Rhein magnetic molecularly imprinted polymer （Fe3O4 @ SiO2 @ MIP） was prepared based on magnetic particles by using rhein as the template molecule, phenyl and amino silanes as binary functional monomers and tetraethyl orthosilicate （TEOS） as cross-linking agent. The Fe3O4 @ SiO2 @ MIP was characterized by SEM and IR. The results showed that rhein imprinted layer was grafted onto the magnetic particles surface successfully. The F%O4@ SiO2@ MIP has uniform cube structure and the thickness of imprinted shell is about 75 - 100 nm. Combined with magnetic solid phase extraction （M-SPE） and HPLC technology, the imprinted material employed as stationary phase was used to separate, enrich and detect rhein, emodin, chrysophanol and physcion from kiwi fruit root extractives successfully.

关 键 词：磁性印迹聚合物 大黄酸 磁固相萃取技术 

分 类 号：O631.3[理学—高分子化学]