贵州5种蝾螈几种组织中乳酸脱氢酶同工酶(LDH)的电泳分析  

Comparative studies on LDH isozyme patterns in some tissues of fives salamander species in Guizhou

作　　者：谷晓明[1] 田应洲 

机构地区：[1]贵州师范大学生物科学技术系,贵州贵阳550001 [2]六盘水高等师范专科学校生物系,贵州水城553001

出　　处：《贵州师范大学学报（自然科学版）》2000年第3期35-37,共3页Journal of Guizhou Normal University：Natural Sciences

基　　金：贵州省自然科学基金项目!(97133)

摘　　要：采用聚丙烯酰胺垂直板凝胶电泳技术 ,分析了贵州疣螈 ,细痣疣螈 ,无斑肥螈 ,蓝尾蝾螈和尾斑瘰螈若干种组织的LDH同工酶 ,结果表明 5种蝾螈种属差异明显 :贵州疣螈 ,细痣疣螈的LDH为 5谱带型 ,而无班肥螈 ,蓝尾蝾螈和尾斑瘰螈的LDH则为非五谱带型 ,各种的LDH亚基构成和迁移率均有所不同。依据 5个种的LDH同工酶的种属差异 ,对它们的系统关系进行了讨论。LDH isozyme of five species Salamander in Guizhou were examined by means of polyacrylamide gelelectrophoresis on vertical slab.The results show that LDH isozyme have obvious race differences among the five species and can be used as race genetic mark; LDH of Tylototriton kweichowensis and T.asperrimus are five-band patterns, LDH of Cynops cyanurus, Parawesotriton caudopunctatus and Pachytriton labiatus are not five-band patterns, and the constitution of subunit,rate of flow of their LDH is different. The systematic relationship were discussed through comparation of LDH isozyme of the five species.

关 键 词：贵州疣螈 细痣疣螈 蓝尾瘰螈 LDH同工酶 蝾螈科 

分 类 号：Q959.520.6[生物学—动物学]