环维黄杨星D对大鼠心肌肥厚的保护作用及机制研究  

Effect of Cyclovirobuxinum D on Cardiac Hypertrophy in Rats and Underlying Mechanism

作　　者：周园[1] 吴俊标[1] 周玖瑶[1] 刘爱军[2] 潘立行[1] 陈孝健[1] 

机构地区：[1]广州中医药大学中药学院,广州510006 [2]广州中医药大学基础医学院

出　　处：《中国药师》2012年第9期1215-1218,共4页China Pharmacist

基　　金：广东省中医药管理局课题(编号:2009231)

摘　　要：目的:探讨环维黄杨星D(Cvb-D)对甲状腺素诱导大鼠心肌肥厚的保护作用及机制。方法:除正常对照组外,各组每日均腹腔注射左旋甲状腺素(L-Thy)1 mg.kg-1,连续14 d,制备大鼠心肌肥厚模型。末次给药24 h后处死大鼠,计算大鼠心脏质量指数(全心质量/体质量,HW/BW)和左心室质量指数(左心室质量/体质量,LVW/BW),观察心肌组织病理形态学改变,并测定心肌组织中一氧化氮(NO)、一氧化氮合成酶(NOS)、超氧化物歧化酶(SOD)、丙二醛(MDA)的含量,评价Cvb-D对大鼠心肌肥厚的影响及可能机制。结果:与对照组比较,L-Thy模型组大鼠心脏质量指数明显升高,病理切片可见心肌肥厚改变,心肌NO、NOS和SOD含量显著降低,MDA显著增加。与模型组相比,Cvb-D中、高剂量组(12,24 mg.kg-1)均能明显降低小鼠HW/BW和LVW/BW,病理切片可见损害较模型组减轻,心肌NO、NOS、SOD含量显著升高(P<0.05),MDA含量显著下降(P<0.01)。结论:环维黄杨星D对L-Thy诱导的大鼠心肌肥厚具有一定的保护作用,其作用机制可能与其抗心肌氧化损伤有关。Objective： To investigate the protective effect of cyclovirobuxinum D（Cvb-D） on cardiac hypertrophy induced by L-Thyroxine（L-Thy） in rats.Method： Cardiac hypertrophy model was established by the continuous administration of L-Thy 1 mg·kg-1·d-1 ip for 14 days.The heart weight index,the morphological changes and the content of NO,NOS,SOD and MDA in cardiac muscle were measured to evaluate the effect of Cvb-D on cardiac hypertrophy.Result： In comparison with the control group,the heart indices of L-Thy group,such as HW/BW and LVW/BW,were obviously increased with notable pathologic changes.The content of NO,NOS and SOD was obviously decreased and that of MDA was notably increased.Cvb-D（12 and 24 mg·kg-1） could obviously decrease the heart indices in rats,improve the pathologic damages of cardiac hypertrophy,increase NO,NOS and SOD,and decrease MDA in cardiac muscle.Conclusion： Cvb-D exhibits the protective effect on cardiac hypertrophy induced by L-Thy in rats.The mechanism could be related with its effect of resisting cardiac muscle oxidative harm.

关 键 词：环维黄杨星D 心肌肥厚 氧化损伤 

分 类 号：R285.5[医药卫生—中药学]