SPE-HPLC法测定人血浆中头孢吡肟的浓度  

Determination of Cefepime in Human Plasma by SEP-HPLC

作　　者：彭才勤[1] 张金安[1] 郭均平[1] 

机构地区：[1]武警湖北总队医院,武汉430061

出　　处：《中国药师》2012年第9期1258-1260,共3页China Pharmacist

摘　　要：目的:建立测定人血浆中头孢吡肟浓度的方法。方法:采用固相萃取(SPE)血浆中头孢吡肟。色谱柱为大连依利特Hypersil BDS C18,流动相为甲醇-磷酸盐缓冲液(20∶80),柱温为30℃,流速为1 ml.min-1,检测波长为257 nm,进样量20μl。结果:头孢吡肟检测浓度线性范围为1.0～200 mg.L-1(r=0.999 9);最低检测限为1 mg.L-1;低、中、高3种浓度头孢吡肟的方法回收率分别为118.0%,97.0%,95.0%,日内RSD分别为1.7%,3.6%,2.1%,日间RSD分别为8.2%,4.9%,2.6%。结论:该方法简便、灵敏度高,适用于测定人血浆中头孢吡肟的含量。Objective： To establish a method to determine cefepime in human plasma by SPE-HPLC.Method： Cefepime in plasma was determined by an SPE-HPLC method.The column was an ELITE Hypersil BDS C18 column.The mobile phase consisted of methanol-phosphate buffer solution（20∶ 80）,the column temperature was 30℃,the flow rate was 1.0 ml·min-1,the detection wavelength was 257 nm,and the sample volume was 20 μl.Result： The calibration curve of cefepime was linear within the range of 1.0~200 mg·L-1 with good linearity（r=0.999 9）.The lowest detection limit was 1 mg·L-1.The recovery was 118.0%,97.0% and 95.0% with intra-day RSD of 1.7%,3.6% and 2.1% and inter-day RSD of 8.2%,4.9% and 2.6% for low,medium and high concentration,respectively.Conclusion： The method is simple and sensitive,and can be used in the determination of cefepime in human plasma.

关 键 词：固相萃取 高效液相色谱法 头孢吡肟 血浆浓度 

分 类 号：R969.1[医药卫生—药理学]