凡纳滨对虾不同类型血细胞的活性氧水平和酯酶活性  

Reactive Oxygen Species(ROS) Production and Esterase Activity in Different Subpopulations of Litopenaeus vnanamei Haemocyte

作　　者：冼健安[1] 王安利[1] 李彬[1] 郭慧[1] 苗玉涛[1] 廖绍安[1] 叶超霞[1] 

机构地区：[1]华南师范大学生命科学学院,广东省水产健康安全养殖重点实验室,生态与环境科学广东普通高校重点实验室,广东广州510631

出　　处：《水生态学杂志》2012年第4期132-137,共6页Journal of Hydroecology

基　　金：国家自然科学基金项目(30671628;31100296);广东省自然科学基金重点项目(S2011020003256);广东省教育部产学研结合资助项目(2011B090500009);广东省科技计划项目(2009A020101006;2011B020307010);广东省重点实验室建设项目(2009A060800019);广州市科技计划项目(2009Z1-E681;11A82090870);中国博士后科学基金项目(2012M511829)

摘　　要：应用流式细胞术(FCM)测定凡纳滨对虾(Litopenaeus vannamei)不同类型血细胞(透明细胞、小颗粒细胞和大颗粒细胞)的非诱导性和诱导性活性氧(ROS)含量以及非特异性酯酶活性,探讨不同类型血细胞的免疫功能。结果显示,透明细胞的非诱导性ROS含量最低,显著低于小颗粒细胞和大颗粒细胞(P<0.05),大颗粒细胞的非诱导性ROS含量最高;经脂多糖(LPS)刺激后,透明细胞的ROS含量没有显著变化(P>0.05),小颗粒细胞和大颗粒细胞的ROS含量显著提高(P<0.05);大颗粒细胞的酯酶活性显著高于小颗粒细胞和透明细胞(P<0.05)。研究表明,3类血细胞在活性氧含量和酯酶活性的功能上存在差异,两类颗粒细胞的活性氧水平较高,大颗粒细胞的酯酶活性较强。To discuss the immune functions of different subpopulations（hyalinocytes,small granulocytes and large granulocytes） of Litopenaeus vannamei haemocyte,the non-induced and induced reactive oxygen species（ROS） production,and non-specific esterase activity were determined using flow cytometry（FCM）.Results showed that the hyalinocytes were lowest in the content of non-induced ROS,while large granulocytes were highest.After induced by LPS,no significant change of ROS content was observed in the hyalinocytes（P0.05）,but significant increase was foundin small granulocytes and large granulocytes（P0.05）.Esterase activity in large granulocytes was significantly higher than that in small granulocytes and large granulocytes（P0.05）.These results demonstrate that ROS production in small granulocytes and large granulocytes are higher than those in hyalinocytes,and esterase activity is higher in large granulocytes.

关 键 词：凡纳滨对虾 血细胞 活性氧 酯酶 

分 类 号：Q952.5[生物学—动物学]