应用流式细胞仪进行CHO细胞计数及存活率计算  

Application of Flow Cytometer in CHO Cell Counting and Cell Survival Rate Calculation

作　　者：高茜[1] 管莹[1] 米其利[1] 李雪梅[1] 缪明明[1] 夭建华[1] 

机构地区：[1]云南烟草科学研究院,云南昆明650106

出　　处：《化学与生物工程》2012年第10期71-74,共4页Chemistry & Bioengineering

基　　金：中国烟草总公司项目(110200902060);云南省烟草化学创新团队建设项目(2009CI014);云南省应用基础研究项目(2009ZC190M);云南省中烟工业公司项目(2011JC02)

摘　　要：以CHO生物工程细胞为对象,探索了流式细胞仪在细胞计数和细胞存活率计算方面的应用。通过设定侧向角散射SS、电子体积EV及荧光强度FL3等3个参数,编制CHO细胞计数程序,再应用流式细胞仪进行细胞计数和存活率计算,其结果与血球计数板法基本一致,但操作更迅速、SD值更低,说明流式细胞仪法较血球计数板法更高效稳定。流式细胞仪法提高了生物工程的生产效率和毒理学评价的准确性,可应用于大规模细胞实验中。Using CHO bioengineering cell as target, application of flow cytometer in cell counting and cell sur-vival rate calculation was explored in this paper. The results showed that cell counting and survival rate calculation could be accurate by the flow cytometer through the set of three parameters SS, EV, and FL3. Compared with blood cell counting plate method, flow cytometer method was more efficient and stable with faster operation and lower SD value. Therefore, to improve the production efficiency and toxicological evaluation reliability, flow cytome- ter method was recommend to be applied in large scale experiments for cell counting and survival rate calculation.

关 键 词：CHO细胞 流式细胞仪 血球计数板 细胞计数 细胞存活率 

分 类 号：Q813[生物学—生物工程]