HCG联合免疫球蛋白对溴隐亭致大鼠流产模型Th1/Th2型细胞因子的影响  

Effect of HCG combining with immunoglobulin G on maternal-fetal interface's Th1/Th2 type cytokines of aborting rats induced by bromocriptine

作　　者：董艳[1,2] 刘福民[3] 刘小云[3] 冯霞[3] 

机构地区：[1]徐州医学院研究生部 [2]江苏省沭阳县人民医院妇产科,江苏沭阳223600 [3]徐州医学院附属医院妇产科,江苏徐州221002

出　　处：《海南医学院学报》2012年第10期1375-1378,共4页Journal of Hainan Medical University

基　　金：中国高校医学期刊临床专项资金项目(112210341)~~

摘　　要：目的:研究人绒毛膜促性腺激素(HCG)联合免疫球蛋白对溴隐亭致大鼠流产模型蜕膜局部Th1/Th2型细胞因子及妊娠结局的影响。方法:采用孕第6、7、8天大鼠皮下注射溴隐亭0.3mg/kg,诱导SD大鼠流产模型,随机分为4组:即流产模型(A)组、HCG(B)组、HCG联合免疫球蛋白(IVIG)(C)组及正常妊娠(D)组,每组10只,妊娠第14天麻醉处死并观察妊娠结局,取胚胎吸收邻近的蜕膜研磨并裂解,离心后取上清液,-80℃保存。ELISA方法测定标本中肿瘤坏死因子-α(TNF-α)、γ-干扰素(IFN-γ)、白细胞介素-4(IL-4)以及白细胞介素-10(IL-10)的水平。结果:胚胎吸收率:A组明显高于其余3组,B组高于C、D组,差异均有统计学意义(P均<0.05)。Th1型细胞因子(TNF-α、IFN-γ)水平,A组明显高于其余3组,差异有统计学意义(P<0.05);Th2型细胞因子(IL-4、IL-10)水平,A组明显低于其余3组,差异均有统计学意义(P<0.05);IL-10细胞因子水平,B、D组低于C组,差异有统计学意义(P<0.05)。结论:HCG联合免疫球蛋白通过上调蜕膜局部细胞因子IL-10水平来改善胚胎发育,二者具有协同作用。Objective.To investigate the effect of the combined therapy of HCG and human immunoglobulin G on local decidual Th1/Th2 type cytokines of bromocriptine induced aborting rats and its in- fluence on pregnancy＇s outcome. Methods： Rat models were induced by hypodermic injection of bromocrip- tine on the 6th ,7th ,8th day of pregnancy, and then divided into four groups： model group（group A）, HCG group（group B）, HCG and human immunoglobulin G group （group C）; normal pregnant rats（D）, On the 14th day after pregnancy, all rats were sacrificed to observe the fetes. Deciduas near the embryo were col- lected and mingled with the RIPA and PMSF, then grounded in a glass container for centrifugation, the su- pernatant were conserved at -80℃ for further use. The TNF-α. IFN-γ,IL-4 .IL-10＇s level of the supernatant were detected by ELISA method. Result：Fetal absorption rate of group A was significant higher than the other three groups（PG0.05） , the rate of group B was also significant higher than group B and C （P〈0. 05）. Compared with group A, Th1 type cytokines （TNF-α, FN-γ,） were significantly decreased in group B, C and D （P〈0. 05） , while Th2 type cytokines （IL-4 and IL-10） all increased in the three groups（P〈0.5）. Conclusions： The combined therapy of HCG and immunoglobulin G can improve the development of fetus by up-regulating local deeiduas IL-10 levels.

关 键 词：大鼠流产模型 人绒毛膜促性腺激素(HCG) 免疫球蛋白 TH1/TH2型细胞因子 

分 类 号：R332[医药卫生—人体生理学] R714.21[医药卫生—基础医学]