1株不典型非O1群霍乱弧菌的发现及鉴定  

Isolation and identification of an atypical non-O1 Vibrio cholerae

作　　者：吴至成[1] 覃西[1] 伍丽娴[2] 翟英超[1] 王小娟[1] 李影林[2] 

机构地区：[1]海口市海南医学院附属医院检验科,海南海口570102 [2]海口市海南医学院热带医学与检验医学院病原生物学教研室,海南海口571199

出　　处：《中华医院感染学杂志》2012年第19期4422-4424,共3页Chinese Journal of Nosocomiology

摘　　要：目的对1株从肝硬化患者血液中分离的病原菌进行鉴定。方法按霍乱弧菌的鉴定标准和方法进行细菌学表型特征(包括形态学、培养特性、生化特征)、血清学鉴定及16SrDNA序列分析。结果从患者血液中分离获得1株病原菌HN 9837,该菌株的形态学、培养特性、生化特征、血清学诊断和分子生物学诊断结果基本符合非O1群霍乱弧菌定义;但与普通霍乱弧菌在甘露醇发酵及赖氨酸利用、柠檬酸盐利用试验结果存在差异。结论海南省首次在肝硬化患者血液中分离出非O1群霍乱弧菌,该菌为1株不发酵甘露醇、赖氨酸且柠檬酸盐利用试验阴性的不典型霍乱弧菌。OBJECTIVE To identify a strain of pathogenic bacterium isolated from the blood of a cirrhotic patient.METHODS The phenotypic characteristics（including morphology,culture characteristics,and biochemical characteristics） and the serologic characteristics were identified according to the standard analytical protocol for Vibrio cholerae,and the 16S rDNA sequence of the isolate was analyzed.RESULTS There was one strain of pathogen（strain HN 9837） which was isolated from the blood of the patient and resembled V.cholerae in its morphological,cultural,biochemical,and serological characters;remarkably,it showed atypical biochemical characteristics such as negative results by tests for mannitol fermentation,lysine decarboxylase and citrate utilization.CONCLUSION It is the first report in Hainan province that non-O1 V.cholerae is isolated from the blood of a cirrhotic patient.The isolate with the following atypical reactions was shown to be V.cholerae： mannitol negative,lysine decarboxylase negative and citrate negative.

关 键 词：非O1群霍乱弧菌 肝硬化 不典型 鉴定 

分 类 号：R378.3[医药卫生—病原生物学]