不同基因缺失型酵母对全氟辛酸的灵敏性  

Sensitivity of different gene deletion yeast to perfluorooctanoic acid

作　　者：金士威[1] 程巧红[1] 戴和平[2] 

机构地区：[1]武汉工程大学化工与制药学院绿色化工过程教育部重点实验室,湖北武汉430074 [2]中国科学院水生生物研究所淡水生态与生物技术国家重点实验室,湖北武汉430072

出　　处：《武汉工程大学学报》2012年第9期5-8,共4页Journal of Wuhan Institute of Technology

基　　金：国家自然科学基金重点项目(21037004)

摘　　要：针对全氟化合物这类新型的有机污染物,在环境中具有持久性,生物累积性和毒性的问题,以全氟辛酸为模型化合物,以真核生物酵母以及4基因缺失型酵母突变体和5基因缺失型酵母突变体为模式生物,开发便捷、灵敏的毒理学评价方法,探究全氟辛酸对不同基因缺失型酵母的毒性效应,以期建立以酵母为平台的环境污染物快速筛选系统.研究结果表明,5基因缺失型酵母突变体对全氟辛酸较为敏感,可以作为建立环境污染物快速筛选系统的细胞模型.Perfluorinated compounds （PFCs） are a class of emerging organic contaminants with persistent stability, strong bioaceumulation and high toxicity. To develop a convenient, sensitive toxicological evaluation method, perfluorooctanoic acid （PFOA）was used as a model compound and eukaryotie yeast and its 4 genes deletion yeast mutant and 5 genes deletion yeast mutant were used as model organisms. The toxic effects of PFOA on different gene deletion yeast were studied. The results show that 5 genes deletion yeast mutant is more sensitive to PFOA, which can be used as a cell model to establish rapid screening system of environmental pollutants.

关 键 词：基因缺失型酵母突变体 基因缺失型酵母突变体 毒性机理 

分 类 号：X835[环境科学与工程—环境工程]