饮水硼对大鼠肾上腺组织结构的影响  

Effect of drinking boron on microtructure of adrenal gland in rats

作　　者：李升和[1] 王珏[1] 周金星[1] 金光明[1] 顾有方[1] 许万祥[1] 

机构地区：[1]安徽科技学院动物科学学院,凤阳233100

出　　处：《卫生研究》2012年第5期799-804,共6页Journal of Hygiene Research

基　　金：教育部科学技术研究重点项目(No.210095);安徽省自然科学基金(No.090411001);安徽高校省级自然科学研究重点项目(No.KJ2009A026Z);安徽科技学院重点学科基金项目(No.AKXK20101-2)

摘　　要：目的研究饮水添加不同水平硼对大鼠肾上腺的平均质量、器官指数及微细结构的影响。方法选用192只(28±2)天清洁级SD大鼠,分为对照组和40、80、160、320和640mg/L硼染毒组,每组32只,雌雄各半,染毒组饮水添加硼酸,对照组饮用蒸馏水,试验期60天。分别于试验的第30天和60天每组随机取鼠16只,雌雄各8只,解剖取肾上腺称重并取材,石蜡切片,HE染色及嗜铬细胞特染,显微观察与摄影。结果试验30天各染毒组雄鼠的肾上腺平均质量均显著或极显著低于对照组(P<0.05或P<0.01);试验60天640mg/L染毒组雄鼠的肾上腺器官指数显著高于对照组(P<0.05)。40mg/L染毒组肾上腺组织结构较对照组有明显改善,髓质细胞内嗜铬颗粒明显增多;80～640mg/L染毒组肾上腺可见不同程度病理组织学变化,病变程度随硼添加量增大而趋于严重;比较观察发现,肾上腺髓质细胞的损伤先于皮质出现,雄鼠的病变较雌鼠明显。结论饮水添加硼40mg/L对大鼠肾上腺的组织结构有较明显改善作用;添加80～640mg/L硼对肾上腺组织结构则有不同程度损伤,甚至有明显毒性作用。the 60th day of experiment, 16 rats （n = 8, male = female） of each group were selected and made into narcosis with 10% Chloral Hydrate. The adrenal glands were obtained, weighted and fixed after dissection, then the samples were made into paraffin sections, stained with HE stain and chromaffin, observed and photographed by Olympus CH-30 microphotograph system. Results Compared with control group, the average mass of adrenal gland of male rats in each experiment group decreased significantly or most significantly at the 30th day of experiment （P 〈 0.05 or P 〈 0.01 ）, but the index ofadrenal gland of male rats in the group of 640 mg/L boron at 60th day of experiment increased significantly （P 〈 0.05）. Under the microscope, the microstructure of adrenal gland of rats in the group of 40 mg/L boron were better obviously than control group, and the numbers of chromaffin granules in chromaffin cell increased obviously. The histopathological changes of different degree could be observed in the group of 80 to 640 mg/L boron, and they became remarkable with the boron supplementation. By comparative observation, the damage of cells in adrenal medulla appeared ahead of them in adrenal cortex, and the pathological change of adrenal gland in ma^e rats were obvious than female rats. Conclusion Drinking supplemented with 40 mg/L boron could prompt the structure of adrenal gland in rats, but could cause different degree damage, or even obvious toxic effect when the concentration of boron supplementation in drinking from 80 to 640 mg/L.

关 键 词：硼 大鼠 肾上腺 组织结构 

分 类 号：R123.1[医药卫生—环境卫生学] Q593[医药卫生—公共卫生与预防医学]