高效液相色谱法测定血清中的9种多环芳烃  

Determination of nine kinds of polycyclic aromatic hydrocarbons in serum by high performance liquid chromatography

作　　者：丁昌明[1] 郑磊[1] 林少彬[1] 

机构地区：[1]中国疾病预防控制中心环境与健康相关产品安全所,北京100021

出　　处：《卫生研究》2012年第5期850-853,共4页Journal of Hygiene Research

基　　金：国家"十一五"科技支撑基金资助项目(No.2006BAI19B01)

摘　　要：目的建立固相萃取—高效液相色谱同时测定血清中蒽、荧蒽、芘、苯并[a]蒽、苯并[b]荧蒽、苯并[k]荧蒽、苯并[a]芘、苯并[a,h]蒽和苯并[g,h,i]芘9种多环芳烃的高效液相色谱测定方法。方法样品经过C18固相萃取小柱富集、净化,以甲醇洗脱后,采用Waters Symmetry C18色谱柱,以乙腈为流动相A,高纯水为流动相B梯度淋洗,流速为1.0 ml/min,荧光检测器测定,检测波长为Ex=340nm,Em=425nm。结果在给定浓度范围内各多环芳烃的峰面积与浓度具有良好的线性关系。其线性范围为0.10～3.00ng/ml;相关系数为0.9941～0.9999;回收率为87.1%～113.4%;相对标准偏差为2.56%～12.7%;检出限为0.05～0.10ng/ml。结论该方法可用于血清中9种多环芳烃的同时测定。Objective To develop a high performance liquid chromatography （LC） method for simultaneously determination of polycyclic aromatic hydrocarbons （PAHs） in serum. Methods PAHs could be separated completely by using Clscolumn and detected by fluorescence detector at wavelengths （Ex = 340nm, Em = 425 nm）. The mobile phase A was acetonitrile and mobile phase B was water with a flow rate at 1.0mL/min. Results The concentration of PAHs achieved a good linear relation with the peak area. The correlation coefficient was in the range of 0. 9941 - 0. 9999. The recoveries were in the range of 91.4% -99.8% and the RSD were in the range of 2.56% -12.7%. The detection limits were in the range of 0.05 - 0. 10ng/ml. Conclusion The method was simple,rapid,sensitive and accurate,and suitable for the detection of PAHs in serum.

关 键 词：多环芳烃 血清 高效液相色谱 

分 类 号：R994.6[医药卫生—毒理学] R446.11[医药卫生—药学]