丹参素对巨噬细胞肝X受体的活化及胆固醇外流的影响  

The impacts of Danshensu on the activation of liver X receptor and the cholesterol efflux of macrophage

作　　者：钱宗杰[1] 曾秋棠[2] 李全忠[1] 

机构地区：[1]桂林医学院附属医院心内科,广西桂林541000 [2]华中科技大学同济医学院附属协和医院心内科

出　　处：《临床心血管病杂志》2012年第10期778-780,共3页Journal of Clinical Cardiology

基　　金：国家自然科学基金地区基金项目(No:81160489)

摘　　要：目的:研究丹参素对巨噬细胞的肝X受体(LXR)及其下游的目的基因的表达和胆固醇外流的影响,探讨丹参素对LXR信号系统的作用。方法:分离人外周血的单核细胞,并转化为巨噬细胞。在丹参素的作用下,观察巨噬细胞的aopA-I介导的胆固醇外流的变化和LXR及其下游目的基因的mRNA及蛋白的表达。结果:丹参素增强细胞内胆固醇的外流,使胆固醇外流的基因LXRα及其下游的一些目的基因表达上调,并呈剂量依赖性。结论:巨噬细胞在丹参素的作用下,上调LXR及其下游影响胆固醇代谢的目的基因,从而使胆固醇外流增强。Objective：To study the effect of Danshensu on the expression of liver X receptor alfa （LXR） and its target genes and the cholesterol efflux of human macrophages. Method： Human peripheral blood monocytes were separated and translated into macrophages. Macrophages were stimulated with or without Danshensu. Cholesterol efflux mediated by ApoA-I-was detected by liquid scintillation counting method. Reverse transcription-polymerase chain reaction （RT-PCR） was used to detect the mRNA expression of LXRa and its target genes, While the pro- tein expression were determined by Western blotting. Result： Incubation with Danshensu, the cholesterol efflux was increased, and both the mRNA and protein expression of LXRa and its target genes were up-regulated by a dose dependently （5--10/mol · L-1） manner in human monocyte-derived macrophage mediated by apolipoprotein AI. Conclusion：The expression of LXR and its target genes influenced cholesterol metabolism were increased by stimulated with Danshensu in macrophages, resulted in the cholesterol efflux increased.

关 键 词：丹参素 胆固醇外流 巨噬细胞 肝X受体 

分 类 号：R541.4[医药卫生—心血管疾病]