三维回转模拟微重力对拟南芥根尖基因表达谱的影响  

Effects of 3-D Clinostat Induced Microgravity on Gene Expression of Arabidopsis Root Tip

作　　者：李华盛[1] 鹿金颖[1] 刘敏[1,2] 袁辉[1] 

机构地区：[1]中国空间技术研究院空间分子生物学实验室,北京100190 [2]中国科学院遗传与发育生物学研究所,北京100101

出　　处：《航天医学与医学工程》2012年第5期330-334,共5页Space Medicine & Medical Engineering

基　　金：国家自然科学基金资助项目(31070769);国家重点基础研究发展计划(2011CB710902);中国载人航天工程资助项目

摘　　要：目的从分子水平上分析和阐述植物根尖对模拟微重力效应的反应。方法以三维回转仪模拟微重力效应,回转处理7 d大小的拟南芥幼苗15 min。利用基因芯片技术,分析模拟微重力下拟南芥mR-NA表达谱的变化。结果拟南芥根尖细胞有392个基因的表达发生了显著变化,其中347个基因表达上调,45个基因表达发生下调。结论模拟微重力效应可以影响拟南芥根尖基因的表达,这些差异表达基因的功能涉及到植物的许多生命过程,包括抗逆反应、细胞壁物质合成、基因转录和信号转导等。Objective To analyze the responses of Arabidopsis root tip to microgravity stimulation from the cellu- lar level. Methods Seven-day old Arabidiosis seedlings were treated with the 3-D elinostat for 15 min to simu- late the microgravity. The mRNA expression profile of Arabidopsis root tip after simulated mierogravity was an- alyzed with microarray. Results Three hundred and thirty-two gene expressions changed signifieantly including 347 up-regulated genes and 45 down-regulated genes. Conclusion Microgravity ean affect the gene expression of Arabidopsis root tip. The function of these genes involve in many life proeesses of plants ineluding stress re- sistance response, cell wall material synthesis, gene transcription, protein synthesis and signal transduetion. Key words ： simulated microgravity ;Arabidopsis ; root tip ; microarray ; gene expression

关 键 词：模拟微重力 拟南芥 根尖 芯片 基因表达 

分 类 号：Q693[生物学—生物物理学]