红毛五加有效成分的抗炎机制初步研究  

Anti-inflammatory mechanism of the Acanthopanax giraldii Harms extract

作　　者：欧阳资章[1] 龙启才[2] 

机构地区：[1]广东省清远市人民医院药事管理科,广东清远511515 [2]中山大学药学院临床药理学教研室,广东广州510080

出　　处：《中国医药科学》2012年第13期23-25,共3页China Medicine And Pharmacy

摘　　要：目的对红毛五加茎皮中有效成分的抗炎机制进行初步研究。方法以LPS刺激小鼠巨噬细胞样细胞RAW264.7,测定红毛五加萃取物氯仿洗脱部分对细胞上清液PGE2、TNF-α、NO、IL-10水平的影响。结果纯氯仿洗脱部分40mg/L剂量组产生的PGE2为(79.57±12.50)pg/mL,TNF-α为(291.6±40.7)pg/mL,NO为(26.2±2.9)μmol,与LPS刺激组相比,差异均有统计学意义。10mg/L剂量组产生的PGE2为(95.20±11.20)pg/mL,TNF-α为(358.6±37.9)pg/mL,NO为(28.3±3.2)μmol,与LPS刺激组相比,差异均有统计学意义。纯氯仿洗脱部分40mg/L剂量组、10mg/L剂量组产生的IL-10分别为(229.4±39.0)pg/mL、(243.7±47.7)pg/mL,与LPS刺激组相比,无统计学意义。结论红毛五加正丁醇萃取部分经氯仿洗脱部分能够抑制炎症因子PGE2、TNF-α、NO的产生而发挥抗炎作用。Objective To investigate the mechanism of the anti-inflammatory effects of the chloroform eluting parts from Acanthopanax giraldii Harms Extract. Methods RAW264.7 cells were stimulated by LPS. The chloroform eluting parts were added to incubate for 20 hours. PGE2, TNF- ct and IL-IO in the supernatants were detected by RIA or Enzyme Line Immunity Assay. NO in the supernatants was detected by Greiss regent kit. Results PGE2 production were （79.57± 12.50） pg/mL and （95.20± 11.20） pg/mL in the group of the chloroform eluting parts （40mg/L） and in the dosage group （10 mg/L）. The chloroform eluting parts inhibited the up-regulation of LPS-induced PGE2 production （P 〈 0.05）. TNF-α production were （291.6 ±40.7） pg/mL and （358.6± 37.9） pg/mL in the dosage group （40 mg/L or 10 mg/L）.The chloroform eluting parts inhibited the up-regulation of LPS-induced TNF-α production （P 〈 0.01）. There were no difference between the group stimulated by LPS and the group of the chloroform eluting parts（P 〉 0.05）. NO production were （26.2 ± 2.9） μ mol/L and （28.3 ± 3.2） μ mol/L in the dosage group （40 mg/L or 10 mg/L）. The chloroform eluting parts inhibited the up-regulation of LPS-induced NO production （P 〈 0.01）. Conclusion The mechanism of anti-inflammatory effects of Acanthopanax giraldii Harms were to inhibit the activity of COX-2 directly and the production of TNF-α and NO in inflammatory cell, but there was no effect to inhibit the production of IL-10.

关 键 词：红毛五加 前列腺素E2 炎症因子 

分 类 号：R927.2[医药卫生—药学]