无偿献血者HIV-1抗体蛋白印迹确认与核酸检测结果分析  

Analyses of the Results of HIV-1 Western Blot Confirmative Test and NAT Test in Voluntary Blood Donors

作　　者：吕蓉[1] 王伦善[1] 盛琪琪[1] 赵阳[1] 蒋菲菲[1] 李敏[1] 刘忠[1] 

机构地区：[1]安徽省血液中心,合肥230031

出　　处：《临床输血与检验》2012年第4期308-311,共4页Journal of Clinical Transfusion and Laboratory Medicine

摘　　要：目的对酶联免疫检测HIV抗体呈反应性标本进行蛋白印迹(WB法)确认和TMA-化学发光法对照检测,以探讨其应用特点。方法将本中心检验科ELISA法检测结果呈HIV抗体反应性的117份标本,重新进行ELISA法检测,结果为S/CO>0.8的标本做蛋白印迹(WB法)确认试验。同时,对117份标本采用TMA-化学发光法检测核酸作为对照试验。血清学检测参加国家CDC及澳大利亚(CITIC)室间质评;核酸检测参加卫生部临检中心和澳大利亚(CITIC)室间质评。结果 ELISA初筛试验:117份标本中,S/CO>1的为37份;0.8<S/CO<1的为11份,S/CO<0.8的为69份;抗体确认试验:HIV抗体确认阳性7份,不确定4份,HIV抗体确认阴性106份;核酸检测试验:117份标本中11份检出HIV RNA阳性,106份标本为HIV RNA阴性。结论 ELISA方法检测HIV抗体存在一定的假阳性;HIV蛋白印迹确认试验存在一些不确定结果,对于只有gp160/120、P17、P24的抗体不确定标本,可以采用核酸检测方法辅助确认。Objective To explore the performance of confirmative Western blot （WB） and TMA-chemical lumines- cence test in detecting the reactive samples for HIV antibody ELISA assay. Methods 117 reactive specimens detected by ELISA were repeatedly tested by two methods of primary screening assay and TMA-luminescence nucleic acid amplification testing（NAT）. All samples of S/CO^O. 8 were analysed by the WB confirmative test. Serological tests participated in the external quality assessment （EQA） were surported by China CDC and Australian international CITIC as well as NAT par- ticipated in the EQA of Clinical Laboratory Center of the Ministry of Health and Australian CITIC. Results The results of all 117 tested samples were as followed, in primary screening test：S/CO〉l,O. 8~S/C0~1 and S/CO〈O. 8 were 37, 11 and 69 cases, respectively. HIV conformative WB test： 7 were positive and 106 were negative, 4 cases could not be de- termined by this method. NAT test： 11 were HIV RNA positive and 106 were negative. Conclusion ELISA assay for the detection of HIV antibodies may result in false positive results. WB method can bring uncertain results. For the uncertain specimens, for instance, only positive in gpl60/1Z0, P17, P24, NAT method can be used to confirm the HIV infection.

关 键 词：HIV-1 蛋白印迹 抗体 核酸检测 

分 类 号：R193.3[医药卫生—卫生事业管理] R373[医药卫生—公共卫生与预防医学]