木质纤维素酶高产菌株的筛选及其对玉米秸秆降解效果的影响  

Selection of ligninase-producing microbes and analysis of their effects on corn stalk degradation

作　　者：郭红伟[1] 王娇[1] 常娟[1] 尹清强[1,2] 左瑞雨[1] 郑秋红[1] 王平 王潇 刘俊熙 

机构地区：[1]河南农业大学牧医工程学院,郑州450002 [2]河南省饲料微生物工程技术研究中心,周口466000

出　　处：《生物加工过程》2012年第5期39-43,共5页Chinese Journal of Bioprocess Engineering

摘　　要：为了提高玉米秸秆中木质素的降解率,从腐烂的树枝和土壤中筛选木质纤维素酶高产菌株,以秸秆为唯一C源富集培养后,采用PDA-愈创木酚法进行初筛,筛选出产木质素酶真菌5株,然后以玉米秸秆为主要C源进行固态发酵和复筛。结果表明:第5号菌株在发酵玉米秸秆5 d后,使木质素的降解率达到最高(34.95%),粗纤维的降解率达到20.00%,显著高于其他4种菌株(P<0.05),其羧甲基纤维素酶比酶活达到116.35 U/g;10 d后,其木质素酶比酶活达到最高(45.64 U/g)。In order to increase corn stalk degradation, five strains of ligninase-producing microbes were selected from rotten plants and soil with the guaiacol as the indicator. The results showed that the fifth strain could produce CMCase at 116.35 U/g, degradation rates of lignin and crude fiber reached 34.95% and 20.00% （P 〈 0.05 ） after 5 d solid-state fermentation. The ligninase activity after 10 d fer mentation medium reached 45.64 U/g.

关 键 词：木质素酶 玉米秸秆 降解率 

分 类 号：TQ352[化学工程]