胶质瘤U251干细胞的培养、分离以及体内成瘤的实验研究  

Experimental Study on Culture,Isolation,and in Vivo Tumorigenicity of Glioma U251 Stem Cells

作　　者：杨德标[1] 邓兴力[1] 杨智勇[1] 黄芩[2] 张兴逵 谢文友[1] 

机构地区：[1]昆明医科大学第一附属医院神经外科,云南昆明650032 [2]昆明医科大学,云南昆明650031

出　　处：《昆明医科大学学报》2012年第9期21-25,共5页Journal of Kunming Medical University

基　　金：云南省专项联合基金资助项目(40209014)

摘　　要：目的以U 251为研究对象探索恶性胶质瘤的新治疗方案,培养恶性胶质瘤干细胞并同时建立肿瘤模型,为下一步体内靶向治疗奠定肿瘤模型基础.方法培养普通的U251细胞,并在无血清的条件下加入EGF、bFGF、LIF、B27因子,培养获得U251干细胞;分别制备浓度为3.5×107/mL的U251和U251干细胞悬液,并分别种植于BALB/c Nude小鼠左右臀部皮下,观察并测量荷瘤体积.结果通过以上方法培养,获得U251干细胞;在相同浓度的条件下,干细胞移植组荷瘤生长速度明显比普通的恶性胶质瘤快,并且荷瘤体积明显大于普通的U251细胞组,差异有统计学意义(P<0.05).结论 U251恶性胶质瘤干细胞比普通U251胶质瘤细胞更具有高生长率、高侵袭性、和高表达CD133抗原;应用U251恶性胶质瘤干细胞建立肿瘤模型更优于普通U251胶质瘤细胞.Objective To explore new treatments for malignant glioma U-251 by culturing malignant glioma stem cells and establishing the tumor model,so as to laid the basis of tumor models for the next step in vivo targeted therapy.Methods We cultured normal U251 cells,and added EGF,bFGF,LIF and B27 factor in serum-free conditions to culture U251 stem cells.U251 and U251 stem cell suspension in 3.5×107/mL were prepared,and were grown in BALB/c Nude left and right hips subcutaneous,then the volume of the tumor-bearing was measured.Results Through the above culture,we obtained U251 stem cells.In the same concentration conditions,the stem cell transplantation group bearing tumor growth rate significantly faster than the ordinary malignant glioma,and tumor-bearing volume was significantly greater than the normal U251 cells group（P0.05）.Conclusions U251 malignant glima stem cell has higher growth rate,invasive ability and CD133 expression level than normal U251 glima cells.The tumor model established by U251 stem cells is better than that by normal U251 cells.

关 键 词：干细胞 U251 恶性胶质瘤 荷瘤体 

分 类 号：Q279[生物学—细胞生物学]