木贼有效部位提取物对氧化低密度脂蛋白诱导内皮细胞凋亡相关基因的调控  

Regulation of effective components in Equisetum on apoptosis-related genes in cultured endothelial cells induced by ox-LDL

作　　者：李志永 王旭辉[2] 李奕[3] 张莉[4] 侯建明[5] 甄艳军[5] 

机构地区：[1]徐水县人民医院病理科,河北保定072550 [2]北京体育大学 [3]中国戏曲学院 [4]河北省儿童医院 [5]河北医科大学基础课教学部

出　　处：《中国老年学杂志》2012年第19期4186-4188,共3页Chinese Journal of Gerontology

基　　金：河北省科技支撑计划项目(06276102D-28)

摘　　要：目的提取木贼有效部位,并观察其对氧化低密度脂蛋白(ox-LDL)损伤人脐静脉内皮细胞(HUVECs)Caspase-3、Bax、Bcl-2 mRNA表达的影响,探讨其保护血管内皮,抗AS(AS)的机制。方法乙醇回流提取、大孔树脂分离纯化出木贼有效部位,并测定其中槲皮素与阿魏酸的含量;ox-LDL刺激培养的HUVECs;反转录聚合酶链反应(RT-PCR)检测内皮细胞Caspase-3、Bax、Bcl-2的mRNA表达。结果木贼有效部位槲皮素与阿魏酸的平均含量分别为1.69,1.23 mg/g,纯度为63.28%;木贼有效部位组能明显降低内皮细胞Caspase-3及Bax mRNA的表达(P<0.01),升高Bcl-2 mRNA的表达(P<0.01),明显降低Bax/Bcl-2比值(P<0.01)。结论所得木贼提取物达到有效部位标准,并通过调节凋亡相关基因抑制ox-LDL诱导的内皮细胞凋亡。Objective To extract the effective components in Equisetum, observe its effect on Caspase-3, Bax, Bcl-2 mRNA ex- pression in cultured human umbilical vein endothelial cells （HUVECs） induced by oxidized low density lipoprotein （ox-LDL） and discuss the mechanism of protective vascular endothelium and anti-atherosclerosis （AS）. Methods Equisetum was extracted by ethanol. After fil- trating it, the macro porous resin was used to isolate and purify the Equisetum extract. The content of Quercetin and ferulic acid in extract were determined by ultraviolet absorption spectrophotometer. HUVECs were cultured in vitro and divided into three groups, then stimulated by ox-LDL（60μg/ml）. The expressions of Caspase-3, Bax, Bcl-2 mRNA were assayed by RT-PCR. Results The total Quercetin average level was 1.69ng/g, the total ferulic acid average level was 1.23 mg/g. The purity of Equisetum effective components was 63.28%. Com- pared with the model group, the expression of Caspase-3 and Bax mRNA in Equisetum effective components group were significantly lower （P 〈 0. 01 ）, the expression of Bcl-2 mRNA was significantly increased （ P 〈 0. 01 ） , Bax/Bcl-2 ratio evident lower （ P 〈 0. 01 ）. Conclu- sions The purity of the extract reaches the standards as effective components. The flavonoids and phenolic acids in Equisetum may play an important role in inhibition of apoptosis through regulating the apoptosis-related genes Caspase-3, Bax, Bcl-2 in HUVECs induced by ox- LDL.

关 键 词：木贼 有效部位 内皮细胞 氧化低密度脂蛋白 凋亡相关基因 

分 类 号：R285.5[医药卫生—中药学]